Abstract
Guidance molecules have attracted interest by demonstration that they regulate patterning of the blood vascular system during development. However, their significance during postnatal angiogenesis has remained unknown. Here, we demonstrate that endothelial cells of human malignant brain tumors also express guidance molecules, such as EphB4 and its ligand ephrinB2. To study their function, EphB4 variants were overexpressed in blood vessels of tumor xenografts. Our studies revealed that EphB4 acts as a negative regulator of blood vessel branching and vascular network formation, switching the vascularization program from sprouting angiogenesis to circumferential vessel growth. In parallel, EphB4 reduces the permeability of the tumor vascular system via activation of the angiopoietin-1/Tie2 system at the endothelium/pericyte interface. Furthermore, overexpression of EphB4 variants in blood vessels during (i) vascularization of non-neoplastic cell grafts and (ii) retinal vascularization revealed that these functions of EphB4 apply to postnatal, non-neoplastic angiogenesis in general. This implies that both neoplastic and non-neoplastic vascularization is driven not only by a vascular initiation program but also by a vascular patterning program mediated by guidance molecules.
MeSH Terms
Angiopoietin-1/metabolism
Animals
Brain Neoplasms/blood supply,metabolism
Capillary Permeability
Cell Line
Endothelial Cells/metabolism
Ephrin-B2/biosynthesis
Glioma/blood supply,metabolism
Humans
Mice
Mice, Inbred C57BL
Mice, Nude
Morphogenesis
Neoplasm Transplantation
Neovascularization, Pathologic
Neovascularization, Physiologic
Receptor, EphB4/biosynthesis,physiology
Receptor, TIE-2/metabolism
Retinal Vessels/growth & development
Signal Transduction
Transplantation, Heterologous
Chemicals
Angiopoietin-1
Ephrin-B2
Receptor, EphB4
Receptor, TIE-2
Authors & Affiliations
10 authors, click to expand affiliations / ORCID
Erber Ralf
Department of Neurosurgery, Faculty for Clinical Medicine of the University of Heidelberg, University Hospital Mannheim, Mannheim, Germany.
Eichelsbacher Uta
Powajbo Violetta
Korn Tobias
Djonov Valentin
Lin Jihong
Hammes Hans-Peter
Grobholz Rainer
Ullrich Axel
Vajkoczy Peter
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