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PMID: 16418482 Published · ppublish English Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't

Quantitative microarray profiling provides evidence against widespread coupling of alternative splicing with nonsense-mediated mRNA decay to control gene expression.

Genes & development ·Vol. 20 ·No. 2 ·2006-01-15 ·Pages 153-8

Pan Q, Saltzman AL, Kim YK, Misquitta C, Shai O, Maquat LE, Frey BJ, Blencowe BJ

Abstract

Sequence-based analyses have predicted that approximately 35% of mammalian alternative splicing (AS) events produce premature termination codon (PTC)-containing splice variants that are targeted by the process of nonsense-mediated mRNA decay (NMD). This led to speculation that AS may often regulate gene expression by activating NMD. Using AS microarrays, we show that PTC-containing splice variants are generally produced at uniformly low levels across diverse mammalian cells and tissues, independently of the action of NMD. Our results suggest that most PTC-introducing AS events are not under positive selection pressure and therefore may not contribute important functional roles.

MeSH Terms
Alternative Splicing Animals Codon, Nonsense/genetics,metabolism Computational Biology Gene Expression Regulation HeLa Cells Humans Mice Oligonucleotide Array Sequence Analysis Open Reading Frames RNA Helicases/metabolism RNA Stability RNA, Messenger/genetics,metabolism Trans-Activators Transfection
Chemicals
Codon, Nonsense RNA, Messenger Rent1 protein, mouse Trans-Activators RNA Helicases UPF1 protein, human
Authors & Affiliations
8 authors, click to expand affiliations / ORCID
Pan Qun
Banting and Best Department of Medical Research, University of Toronto, Ontario, M5G 1L6, Canada.
Saltzman Arneet L
Kim Yoon Ki
Misquitta Christine
Shai Ofer
Maquat Lynne E
Frey Brendan J
Blencowe Benjamin J
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Article Info
Journal
Genes & development
Abbr.
Genes Dev
ISSN
0890-9369
DOI
10.1101/gad.1382806
Published
2006-01-15
Pages
153-8
Language
English
Region
United States
NLM ID
8711660
PMCID
PMC1356107
Subset
IM
Grants
NIGMS NIH HHS · R01 GM059614 · United States
NIGMS NIH HHS · GM059614 · United States
External Links
PubMed source DOI: 10.1101/gad.1382806 PMC Full Text View journal details
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