Light-stimulated phosphorylation of rhodopsin in the retina: the presence of a protein kinase that is specific for photobleached rhodopsin.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 72 ·No. 1 ·1975-01-00 ·Pages 381-5

Weller M, Virmaux N, Mandel P

Abstract

A protein kinase has been extracted from bovine rod outer segments by a mild procedure. The enzyme acts specifically on photobleached, not unbleached, rhodopsin and will not catalyze the phosphorylation of histones, phosvitin, or casein. We propose the name "opsin kinase" for the enzyme, which is not affected by cyclic nucleotides but which is inhibited by theophylline. Preparations of purified rod outer segments, however, appear to contain only low concentration of opsin phosphatase activity.

MeSH Terms

Adenosine Triphosphate/metabolism Animals Caseins/metabolism Cattle Cyclic AMP/pharmacology Cyclic GMP/pharmacology Enzyme Activation/drug effects Histones/metabolism Light Phosphates/metabolism Phosphodiesterase Inhibitors Phosphorus Radioisotopes Photic Stimulation Photoreceptor Cells/enzymology,metabolism Protein Kinases/metabolism Retinal Pigments/metabolism Rhodopsin/metabolism Theophylline/pharmacology

Chemicals

Caseins Histones Phosphates Phosphodiesterase Inhibitors Phosphorus Radioisotopes Retinal Pigments Adenosine Triphosphate Rhodopsin Theophylline Cyclic AMP Protein Kinases Cyclic GMP

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Weller M

Virmaux N

Mandel P

References (25)

25 references, click to expand

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Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.72.1.381

Published

1975-01-00

Pages

381-5

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC432309

Subset

External Links

PubMed source DOI: 10.1073/pnas.72.1.381 PMC Full Text View journal details

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Light-stimulated phosphorylation of rhodopsin in the retina: the presence of a protein kinase that is specific for photobleached rhodopsin.

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