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PMID: 164014 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S.

Interferon action II. Membrane-bound alkaline ribonuclease activity in chick embryo cells manifesting interferon-mediated interference.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 72 ·No. 1 ·1975-01-00 ·Pages 182-6

Marcus PI, Terry TM, Levine S

Abstract

Membrane fractions from chick embryo cells manifesting viral interference mediated by interferon or poly(I)-poly(C) contain high levels of an alkaline ribonuclease. Enhanced RNase activity is not observed when inhibitors of cell protein or RNA synthesis are present during interferon treatment, or when heterologous interferon is used. The RNase associated with comparable membrane fractions from cells treated with mock-interferon is about 1/10 as active, and shows qualitative differences. In principle, divergent views of interferon action may be reconciled to a common mode of action by postulating that viral interference results from a newly induced or activated RNase of cellular origin and proper specificity that acts to reduce the accumulation and functional capacity of newly synthesized viral RNAs, particularly mRNA. Previous data in support of interferon's acting to inhibit virion-derived transcription in vivo are now interpreted as demonstrating enhanced degradation of viral transcripts (mRNA).

MeSH Terms
Animals Cell Membrane/enzymology Cells, Cultured Chick Embryo Enzyme Activation/drug effects Enzyme Induction/drug effects Interferons/pharmacology Poly I-C/pharmacology Protein Biosynthesis/drug effects RNA, Messenger/biosynthesis RNA, Viral/biosynthesis Ribonucleases/metabolism Transcription, Genetic/drug effects Vesicular stomatitis Indiana virus/drug effects Viral Interference/drug effects
Chemicals
RNA, Messenger RNA, Viral Interferons Ribonucleases Poly I-C
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Marcus P I
Terry T M
Levine S
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26 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.72.1.182
Published
1975-01-00
Pages
182-6
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC432266
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.72.1.182 PMC Full Text View journal details
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