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PMID: 163921 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Virion polypeptide composition of the human papovavirus BK: comparison with simian virus 40 and polyoma virus.

Journal of virology ·Vol. 15 ·No. 4 ·1975-04-00 ·Pages 828-35

Wright PJ, Di Mayorca G

Abstract

The polypeptide composition of labeled BK virus was compared with that of simian virus 40 (SV40) and polyoma virus by co-electrophoresis of disrupted virions in polyacrylamide gels containing approximately 73% of the capsid protein and had a molecular weight of 39,000. It was smaller than VP1 of SV40 and polyoma virus. The other polypeptides of BK virus were similar in molecular weight to those of SV40. A comparison of the proteins of BK virus and SV40 iodinated with chloramine T before and after disruption in alkaline buffer at pH 10.5 revealed differences between the two viruses in the number and distribution of tyrosines available for iodination. The tryptic peptides of VP1, VP3, VP4, and VP5 combined of SV40 were compared with those of the same polypeptides of BK virus. Among the 19 peptides of VP1 resolved, only two were common to both viruses. The analyses of VP4 and VP5, the histone-like proteins, however, showed more similarity between the viruses, with 6 of 15 resolved peptides in common. The tryptic digests of VP3 were completely different.

MeSH Terms
Carbon Radioisotopes Centrifugation, Zonal Electrophoresis, Polyacrylamide Gel Iodine Lysine Molecular Weight Papillomaviridae/analysis Peptides/analysis Polyomaviridae Polyomavirus/analysis Protein Hydrolysates Simian virus 40/analysis Tritium Trypsin
Chemicals
Carbon Radioisotopes Peptides Protein Hydrolysates Tritium Iodine Trypsin Lysine
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Wright P J
Di Mayorca G
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21 references, click to expand
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.15.4.828-835.1975
Published
1975-04-00
Pages
828-35
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC354526
Subset
IM
External Links
PubMed source DOI: 10.1128/JVI.15.4.828-835.1975 PMC Full Text View journal details
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