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PMID: 16385064 Published · ppublish English Journal Article

DNA sequence of a ColV plasmid and prevalence of selected plasmid-encoded virulence genes among avian Escherichia coli strains.

Journal of bacteriology ·Vol. 188 ·No. 2 ·2006-01-00 ·Pages 745-58

Johnson TJ, Siek KE, Johnson SJ, Nolan LK

Abstract

ColV plasmids have long been associated with the virulence of Escherichia coli, despite the fact that their namesake trait, ColV production, does not appear to contribute to virulence. Such plasmids or their associated sequences appear to be quite common among avian pathogenic E. coli (APEC) and are strongly linked to the virulence of these organisms. In the present study, a 180-kb ColV plasmid was sequenced and analyzed. This plasmid, pAPEC-O2-ColV, possesses a 93-kb region containing several putative virulence traits, including iss, tsh, and four putative iron acquisition and transport systems. The iron acquisition and transport systems include those encoding aerobactin and salmochelin, the sit ABC iron transport system, and a putative iron transport system novel to APEC, eit. In order to determine the prevalence of the virulence-associated genes within this region among avian E. coli strains, 595 APEC and 199 avian commensal E. coli isolates were examined for genes of this region using PCR. Results indicate that genes contained within a portion of this putative virulence region are highly conserved among APEC and that the genes of this region occur significantly more often in APEC than in avian commensal E. coli. The region of pAPEC-O2-ColV containing genes that are highly prevalent among APEC appears to be a distinguishing trait of APEC strains.

MeSH Terms
Animals Carrier State/microbiology Chickens/microbiology DNA, Bacterial/genetics Escherichia coli/genetics,metabolism Escherichia coli Infections/microbiology,veterinary Genes, Bacterial Iron/metabolism Plasmids/genetics Poultry Diseases/microbiology Virulence Factors/genetics
Chemicals
DNA, Bacterial Virulence Factors Iron
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Johnson Timothy J
Department of Veterinary Microbiology and Preventive Medicine, College of Veterinary Medicine, 1802 Elwood Drive, VMRI #2, Iowa State University, Ames, IA 50011. lknolan@iastate.edu.
Siek Kylie E
Johnson Sara J
Nolan Lisa K
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/JB.188.2.745-758.2006
Email
lknolan@iastate.edu
Published
2006-01-00
Pages
745-58
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC1347294
Subset
IM
External Links
PubMed source DOI: 10.1128/JB.188.2.745-758.2006 PMC Full Text View journal details
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