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PMID: 163800 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Effect of immunosuppression on the genetic resistance of A2G mice to neurovirulent influenza virus.

Infection and immunity ·Vol. 11 ·No. 3 ·1975-03-00 ·Pages 576-87

Fiske RA, Klein PA

Abstract

A2G mice are genetically resistant to lethal infection with neurotropic and pneumotropic influenza viruses. A possible immunological explanation for this resistance was sought by assessing the effect of cyclophosphamide and X irradiation immunosuppression on the infection of A2G mice with lethal doses of neurovirulent virus. Immunosuppressed A2G mice survived lethal infection enen though rendered unable to produce specific antiviral antibody or to generate cell-mediated delayed-type hypersensitivity responses. Measurement of infectious virus replication and detailed observation of the infection by immunofluorescence microscopy show that immunosuppression does not potentiate or allow spread of the virus in A2G brains. Interferon levels were essentially the same in normal and immunosuppressed A2G brains but were 3 to 5 times lower than in the brains of susceptible mice dying of the infection. The results strongly suggest that the genetic resistance of A2G mice to the acute lethal effects of neurovirulent influenza virus infection does not depend on the induction of primary immune mechanisms as we currently understand them. Other possible explanations for this resistance are considered.

MeSH Terms
Animals Antibodies, Viral/analysis Brain/immunology Culture Techniques Cyclophosphamide/pharmacology Female Fluorescent Antibody Technique Hemagglutination Inhibition Tests Hemolysin Proteins/analysis Hypersensitivity, Delayed/immunology Immune Sera Immunity Immunization Immunosuppression Therapy Interferons/analysis Male Mice Mice, Inbred Strains Orthomyxoviridae/immunology Orthomyxoviridae Infections/immunology Rabbits/immunology Radiation Injuries, Experimental Vesicular stomatitis Indiana virus/immunology Viral Plaque Assay
Chemicals
Antibodies, Viral Hemolysin Proteins Immune Sera Cyclophosphamide Interferons
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Fiske R A
Klein P A
References (33)
33 references, click to expand
  1. Prevention of cell-to-cell spread of herpes simplex virus by leukocytes.
    J Exp Med. 1973 Mar 1;137(3):706-20 PMID: 4347596
  2. Murine influenza virus encephalomyelitis. II. Electron microscopic observations.
    J Neuropathol Exp Neurol. 1973 Jan;32(1):72-91 PMID: 4346658
  3. Immune mechanisms by which the spread of viral infections is stopped.
    Cell Immunol. 1974 Mar 30;11(1-3):478-83 PMID: 4376059
  4. Athymic (nude) mice express gene for myxovirus resistance.
    Nature. 1974 Aug 23;250(5468):679-80 PMID: 4546732
  5. Respiratory diseases in cyclophosphamide-treated mice. II. Decreased virulence of PR8 influenza virus.
    Infect Immun. 1972 Jun;5(6):957-60 PMID: 4635505
  6. Nonlinkage of neurovirulence exclusively to viral hemagglutinin or neuraminidase in genetic recombinants of A-NWS (HON1) influenza virus.
    J Virol. 1973 Feb;11(2):272-8 PMID: 4688703
  7. Effects of immunosuppression on coxsackie B-3 virus infection in mice, and passive protection by circulating antibody.
    J Gen Virol. 1973 Jun;19(3):339-51 PMID: 4723533
  8. Pathogenesis for viral infections of the nervous system.
    N Engl J Med. 1968 Jan 11;278(2):84-92 concl PMID: 4870529
  9. Replication of influenza virus in a continuous cell line: high yield of infective virus from cells inoculated at high multiplicity.
    Virology. 1969 Sep;39(1):130-4 PMID: 4980034
  10. Immunosuppression and experimental virus infection of the nervous system.
    Adv Virus Res. 1970;16:397-448 PMID: 4993221
  11. Influenza virus encephalitis in squirrel monkeys receiving immunosuppressive therapy.
    J Immunol. 1971 Apr;106(4):1119-21 PMID: 4995003
  12. Specificity of the inflammatory response in viral encephalitis. I. Adoptive immunization of immunosuppressed mice infected with Sindbis virus.
    J Exp Med. 1972 Aug 1;136(2):216-26 PMID: 5043410
  13. Mechanism of recovery from systemic vaccinia virus infection. I. The effects of cyclophosphamide.
    J Exp Med. 1972 Aug 1;136(2):277-90 PMID: 5043413
  14. Inter- feron synthesis in x-irradiated animals. 3. The high radiosensitivity of myxovirus-induced circulating interferon production.
    Proc Soc Exp Biol Med. 1969 May;131(1):36-41 PMID: 5770121
  15. The structure of influenza viruses. IV. Chemical studies of the host antigen.
    Virology. 1966 Sep;30(1):104-15 PMID: 5911885
  16. Studies on susceptibility to infection following ionizing radiation. I. The time of onset and duration of the endogenous bacteremias in mice.
    J Exp Med. 1954 May 1;99(5):405-10 PMID: 13163317
  17. THE RESISTANCE OF A2G MICE TO MYXOVIRUSES.
    J Immunol. 1963 Jun;90:942-51 PMID: 14050204
  18. INHERITANCE OF RESISTANCE TO INFLUENZA VIRUS IN MICE.
    Proc Soc Exp Biol Med. 1964 Jun;116:506-9 PMID: 14193387
  19. EPIDEMIOLOGICAL ASPECTS OF THE CONTROL OF PSEUDOMONAS INFECTION IN MOUSE COLONIES.
    Lab Anim Care. 1965 Jun;15:217-25 PMID: 14302823
  20. Intracerebral injections and the growth of viruses in the mouse brain.
    Br J Exp Pathol. 1960 Feb;41:52-9 PMID: 14422710
  21. Intracerebral inoculation of mice; fate of the inoculum.
    Nature. 1950 Nov 25;166(4230):910-1 PMID: 14796624
  22. Interactions of antibodies, complement components and various cell types in immunity against viruses and pyogenic bacteria.
    Transplant Rev. 1974;19(0):3-55 PMID: 4135465
  23. Chromatographic purification of tetramethylrhodamine-immune globulin conjugates and their use in the cellular localization of rabbit gamma-globulin polypeptide chains.
    J Immunol. 1965 Aug;95(2):230-45 PMID: 4158328
  24. Contact and delayed hypersensitivity in the mouse. I. Active sensitization and passive transfer.
    Immunology. 1968 Sep;15(3):405-16 PMID: 4177017
  25. Viral oncolysis: increased immunogenicity of host cell antigen associated with influenza virus.
    J Exp Med. 1967 Jul 1;126(1):93-108 PMID: 4290961
  26. Preparation of enriched fractions from cerebral cortex containing isolated, metabolically active neuronal and glial cells.
    Biochem J. 1967 Jan;102(1):33-43 PMID: 4291562
  27. Effects of antilymphocyte serum on virus oncogenesis.
    Proc Soc Exp Biol Med. 1968 Jan;127(1):207-12 PMID: 4296244
  28. Factors modifying host resistance to viral infection. 3. Effect of whole body x-irradiation on experimental encephalomyocarditis virus infection in mice.
    J Exp Med. 1968 May 1;127(5):1035-52 PMID: 4297630
  29. In vitro interaction of mouse hepatitis virus and macrophages from genetically resistant mice. I. Adsorption of virus and growth curves.
    J Exp Med. 1970 Apr 1;131(4):843-50 PMID: 4317219
  30. In vitro interaction of mouse hepatitis virus and macrophages from genetically resistant mice. II. Biological characterization of a variant virus MHV (C3H) isolated from stocks of MHV(PRI).
    J Exp Med. 1970 Apr 1;131(4):851-62 PMID: 4317220
  31. Mechanisms of recovery from a generalized viral infection: mousepox. I. The effects of anti-thymocyte serum.
    J Exp Med. 1970 Nov;132(5):1035-54 PMID: 4319373
  32. Murine influenza virus encephalomyelitis. I. Neuropathological and immunofluorescence findings.
    J Neuropathol Exp Neurol. 1973 Jan;32(1):51-71 PMID: 4346657
  33. Effect of cyclophosphamide on the genetic resistance of C 3 H mice to mouse hepatitis virus.
    Proc Soc Exp Biol Med. 1973 Mar;142(3):762-6 PMID: 4348090
Article Info
Journal
Infection and immunity
Abbr.
Infect Immun
ISSN
0019-9567
DOI
10.1128/iai.11.3.576-587.1975
Published
1975-03-00
Pages
576-87
Language
English
Region
United States
NLM ID
0246127
PMCID
PMC415103
Subset
IM
External Links
PubMed source DOI: 10.1128/iai.11.3.576-587.1975 PMC Full Text View journal details
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