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PMID: 16378242 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Multiple cAMP-induced signaling cascades regulate prolactin expression in T cells.

Cellular and molecular life sciences : CMLS ·Vol. 63 ·No. 1 ·2006-01-00 ·Pages 92-9

Gerlo S, Verdood P, Hooghe-Peters EL, Kooijman R

Abstract

Beside its pivotal role in reproduction, the pituitary hormone prolactin (PRL) has been attributed an immunomodulatory function. Here we report that cAMP is an important stimulator of PRL transcription in primary human T lymphocytes. Inhibition of both protein kinase A (PKA) and p38 MAPK partially abrogated cAMP-induced PRL expression. In addition, cAMP-induced phosphorylation of p38 was shown to occur independently of PKA and could be mimicked by a methylated cAMP analogue which specifically activates the recently discovered cAMP receptor EPAC (exchange protein directly activated by cAMP). Our findings suggest that cAMP induces PRL expression in T lymphocytes via cooperation of at least two different signaling pathways: a PKA-dependent pathway leading to the phosphorylation of cAMP response element-binding protein, and a PKA-independent pathway leading to p38 phosphorylation.

MeSH Terms
Cells, Cultured Cyclic AMP/pharmacology Cyclic AMP-Dependent Protein Kinases/metabolism Gene Expression Regulation/drug effects Guanine Nucleotide Exchange Factors/genetics Humans Mitogen-Activated Protein Kinases/metabolism Prolactin/genetics,metabolism RNA, Messenger/genetics Signal Transduction/drug effects T-Lymphocytes/drug effects,metabolism Transcription, Genetic/genetics
Chemicals
Guanine Nucleotide Exchange Factors RAPGEF3 protein, human RNA, Messenger Prolactin Cyclic AMP Cyclic AMP-Dependent Protein Kinases Mitogen-Activated Protein Kinases
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Gerlo S
Neuroendocrine Immunology Research Group, Department of Pharmacology, Free University of Brussels (VUB), Laarbeeklaan 103, 1090 Brussels, Belgium.
Verdood P
Hooghe-Peters E L
Kooijman R
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Article Info
Journal
Cellular and molecular life sciences : CMLS
Abbr.
Cell Mol Life Sci
ISSN
1420-682X
DOI
10.1007/s00018-005-5433-4
Published
2006-01-00
Pages
92-9
Language
English
Region
Switzerland
NLM ID
9705402
PMCID
PMC2792358
Subset
IM
External Links
PubMed source DOI: 10.1007/s00018-005-5433-4 PMC Full Text View journal details
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