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PMID: 16372010 Published · ppublish English Journal Article

Genome sequencing and analysis of Aspergillus oryzae.

Nature ·Vol. 438 ·No. 7071 ·2005-12-22 ·Pages 1157-61

Machida M, Asai K, Sano M, Tanaka T, Kumagai T, Terai G, Kusumoto K, Arima T, Akita O, Kashiwagi Y, Abe K, Gomi K, Horiuchi H, Kitamoto K, Kobayashi T, Takeuchi M, Denning DW, Galagan JE, Nierman WC, Yu J, Archer DB, Bennett JW, Bhatnagar D, Cleveland TE, Fedorova ND, Gotoh O, Horikawa H, Hosoyama A, Ichinomiya M, Igarashi R, Iwashita K, Juvvadi PR, Kato M, Kato Y, Kin T, Kokubun A, Maeda H, Maeyama N, Maruyama J, Nagasaki H, Nakajima T, Oda K, Okada K, Paulsen I, Sakamoto K, Sawano T, Takahashi M, Takase K, Terabayashi Y, Wortman JR, Yamada O, Yamagata Y, Anazawa H, Hata Y, Koide Y, Komori T, Koyama Y, Minetoki T, Suharnan S, Tanaka A, Isono K, Kuhara S, Ogasawara N, Kikuchi H

Abstract

The genome of Aspergillus oryzae, a fungus important for the production of traditional fermented foods and beverages in Japan, has been sequenced. The ability to secrete large amounts of proteins and the development of a transformation system have facilitated the use of A. oryzae in modern biotechnology. Although both A. oryzae and Aspergillus flavus belong to the section Flavi of the subgenus Circumdati of Aspergillus, A. oryzae, unlike A. flavus, does not produce aflatoxin, and its long history of use in the food industry has proved its safety. Here we show that the 37-megabase (Mb) genome of A. oryzae contains 12,074 genes and is expanded by 7-9 Mb in comparison with the genomes of Aspergillus nidulans and Aspergillus fumigatus. Comparison of the three aspergilli species revealed the presence of syntenic blocks and A. oryzae-specific blocks (lacking synteny with A. nidulans and A. fumigatus) in a mosaic manner throughout the genome of A. oryzae. The blocks of A. oryzae-specific sequence are enriched for genes involved in metabolism, particularly those for the synthesis of secondary metabolites. Specific expansion of genes for secretory hydrolytic enzymes, amino acid metabolism and amino acid/sugar uptake transporters supports the idea that A. oryzae is an ideal microorganism for fermentation.

MeSH Terms

Aspartic Acid Endopeptidases/genetics Aspergillus oryzae/enzymology,genetics,metabolism Chromosomes, Fungal/genetics Cytochrome P-450 Enzyme System/genetics Genes, Fungal/genetics Genome, Fungal Genomics Molecular Sequence Data Phylogeny Synteny

Chemicals

Cytochrome P-450 Enzyme System Aspartic Acid Endopeptidases

Authors & Affiliations

64 authors, click to expand affiliations / ORCID

Machida Masayuki

Institute for Biological Resources and Functions, National Institute of Advanced Industrial Science and Technology (AIST), Higashi 1-1-1, Tsukuba, Ibaraki 305-8566, Japan. m.machida@aist.go.jp

Asai Kiyoshi

Sano Motoaki

Tanaka Toshihiro

Kumagai Toshitaka

Terai Goro

Kusumoto Ken-Ichi

Arima Toshihide

Akita Osamu

Kashiwagi Yutaka

Abe Keietsu

Gomi Katsuya

Horiuchi Hiroyuki

Kitamoto Katsuhiko

Kobayashi Tetsuo

Takeuchi Michio

Denning David W

Galagan James E

Nierman William C

Yu Jiujiang

Archer David B

Bennett Joan W

Bhatnagar Deepak

Cleveland Thomas E

Fedorova Natalie D

Gotoh Osamu

Horikawa Hiroshi

Hosoyama Akira

Ichinomiya Masayuki

Igarashi Rie

Iwashita Kazuhiro

Juvvadi Praveen Rao

Kato Masashi

Kato Yumiko

Kin Taishin

Kokubun Akira

Maeda Hiroshi

Maeyama Noriko

Maruyama Jun-ichi

Nagasaki Hideki

Nakajima Tasuku

Oda Ken

Okada Kinya

Paulsen Ian

Sakamoto Kazutoshi

Sawano Toshihiko

Takahashi Mikio

Takase Kumiko

Terabayashi Yasunobu

Wortman Jennifer R

Yamada Osamu

Yamagata Youhei

Anazawa Hideharu

Hata Yoji

Koide Yoshinao

Komori Takashi

Koyama Yasuji

Minetoki Toshitaka

Suharnan Sivasundaram

Tanaka Akimitsu

Isono Katsumi

Kuhara Satoru

Ogasawara Naotake

Kikuchi Hisashi

Article Info

Journal

Nature

Abbr.

Nature

ISSN

1476-4687

DOI

10.1038/nature04300

m.machida@aist.go.jp

Published

2005-12-22

Pages

1157-61

Language

English

Region

England

NLM ID

0410462

Subset

Grants

Biotechnology and Biological Sciences Research Council · CFB17726 · United Kingdom

Databases

GENBANK

AP007150, AP007151, AP007152, AP007153, AP007154, AP007155, AP007156, AP007157, AP007158, AP007159, AP007160, AP007161, AP007162, AP007163, AP007164, AP007165, AP007166, AP007167, AP007168, AP007169, AP007170, AP007171, AP007172, AP007173, AP007174, AP007175, AP007176, AP007177

Corrections

CommentIn

PMID: 16371993

External Links

PubMed source DOI: 10.1038/nature04300 View journal details

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Genome sequencing and analysis of Aspergillus oryzae.

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