Isolation of Rhizobium meliloti nod Gene Inducers from Alfalfa Rhizosphere Soil.

Applied and environmental microbiology ·Vol. 59 ·No. 2 ·1993-02-00 ·Pages 636-9

León-Barrios M, Dakora FD, Joseph CM, Phillips DA

Abstract

Methanolic extracts of alfalfa rhizosphere soil induce more nod gene transcription in Rhizobium meliloti than extracts of nonrhizosphere soil. Six peaks of nod-inducing activity were separated by high-performance liquid chromatography from rhizosphere soil extract, and one compound was identified by H nuclear magnetic resonance, mass spectrometry, and UV-visible spectra as a formononetin-7-O-glycoside that activates both NodD1 and NodD2 proteins. The unanticipated presence of a glycoside in rhizosphere soil suggests either that large amounts of the glycoside were exuded by roots or that some glycosides are unexpectedly stable in soil.

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

León-Barrios M

Department of Agronomy and Range Science, University of California, Davis, California 95616.

Dakora F D

Joseph C M

Phillips D A

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15 references, click to expand

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Article Info

Journal

Applied and environmental microbiology

Abbr.

Appl Environ Microbiol

ISSN

0099-2240

DOI

10.1128/aem.59.2.636-639.1993

Published

1993-02-00

Pages

636-9

Language

English

Region

United States

NLM ID

7605801

PMCID

PMC202160

External Links

PubMed source DOI: 10.1128/aem.59.2.636-639.1993 PMC Full Text View journal details

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Isolation of Rhizobium meliloti nod Gene Inducers from Alfalfa Rhizosphere Soil.

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