Ethanol extraction requirement for purification of protein labeled with [h]leucine in aquatic bacterial production studies.

Applied and environmental microbiology ·Vol. 54 ·No. 12 ·1988-12-00 ·Pages 3191-3

Wicks RJ, Robarts RD

Abstract

The trichloroacetic acid (TCA)-insoluble fraction of water column bacteria labeled with [H]leucine contained an ethanol-soluble fraction accounting for up to 44% of the label. A component of the ethanol-soluble fraction is [H]leucine. Labeled-protein purification requires an ethanol wash step. Cold TCA can replace hot TCA for precipitation of labeled proteins.

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Wicks R J

Division of Water Technology, Council for Scientific and Industrial Research, P. O. Box 395, Pretoria 0001, South Africa.

Robarts R D

References (4)

4 references, click to expand

Mechanisms of heat damage in proteins. 8. The role of sucrose in the susceptibility of protein foods to heat damage.
Br J Nutr. 1977 Sep;38(2):285-97 PMID: 20923
Estimating bacterial production in marine waters from the simultaneous incorporation of thymidine and leucine.
Appl Environ Microbiol. 1988 Aug;54(8):1934-9 PMID: 16347706
Spatial and Temporal Variations in Bacterial Macromolecule Labeling with [methyl-H]Thymidine in a Hypertrophic Lake.
Appl Environ Microbiol. 1986 Dec;52(6):1368-73 PMID: 16347241
Leucine incorporation and its potential as a measure of protein synthesis by bacteria in natural aquatic systems.
Appl Environ Microbiol. 1985 Mar;49(3):599-607 PMID: 3994368

Article Info

Journal

Applied and environmental microbiology

Abbr.

Appl Environ Microbiol

ISSN

0099-2240

DOI

10.1128/aem.54.12.3191-3193.1988

Published

1988-12-00

Pages

3191-3

Language

English

Region

United States

NLM ID

7605801

PMCID

PMC204452

External Links

PubMed source DOI: 10.1128/aem.54.12.3191-3193.1988 PMC Full Text View journal details

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Ethanol extraction requirement for purification of protein labeled with [h]leucine in aquatic bacterial production studies.

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