Identification of lotus rhizobia by direct DNA hybridization of crushed root nodules.

Applied and environmental microbiology ·Vol. 53 ·No. 7 ·1987-07-00 ·Pages 1705-7

Cooper JE, Bjourson AJ, Thompson JK

Abstract

Hybridization of crushed Lotus pedunculatus root nodules with P-labeled total genomic DNA probes was used to identify Rhizobium loti and Bradyrhizobium sp. (Lotus rhizobia). Probes always hybridized with homologous target DNA and frequently with DNAs of other strains from the same genus. Intergeneric hybridization did not occur. Results were comparable to those from colony hybridization.

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Cooper J E

Agricultural and Food Bacteriology Research Division, Department of Agriculture for Northern Ireland, Belfast BT9 5PX, Northern Ireland.

Bjourson A J

Thompson J K

References (4)

4 references, click to expand

Colony hybridization: a method for the isolation of cloned DNAs that contain a specific gene.
Proc Natl Acad Sci U S A. 1975 Oct;72(10):3961-5 PMID: 1105573
C(4)-dicarboxylate transport mutants of Rhizobium trifolii form ineffective nodules on Trifolium repens.
Proc Natl Acad Sci U S A. 1981 Jul;78(7):4284-8 PMID: 16593058
Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I.
J Mol Biol. 1977 Jun 15;113(1):237-51 PMID: 881736
DNA-DNA hybridization assay for detection of Salmonella spp. in foods.
Appl Environ Microbiol. 1983 Nov;46(5):1146-51 PMID: 6360046

Article Info

Journal

Applied and environmental microbiology

Abbr.

Appl Environ Microbiol

ISSN

0099-2240

DOI

10.1128/aem.53.7.1705-1707.1987

Published

1987-07-00

Pages

1705-7

Language

English

Region

United States

NLM ID

7605801

PMCID

PMC203937

External Links

PubMed source DOI: 10.1128/aem.53.7.1705-1707.1987 PMC Full Text View journal details

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Identification of lotus rhizobia by direct DNA hybridization of crushed root nodules.

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