Construction of Streptococcus lactis subsp. lactis Strains with a Single Plasmid Associated with Mucoid Phenotype.

Applied and environmental microbiology ·Vol. 53 ·No. 6 ·1987-06-00 ·Pages 1385-6

von Wright A, Tynkkynen S

Abstract

Lactose-fermenting mucoid (Lac Muc) variants of plasmid-free Streptococcus lactis subsp. lactis MG1614 were obtained by protoplast transformation with total plasmid DNA from MucS. lactis subsp. cremoris ARH87. By using plasmid DNA from these variants for further transformations followed by novobiocininduced plasmid curing, Lac Muc MG1614 strains containing only a single 30-megadalton plasmid could be constructed. This plasmid, designated pVS5, appeared to be associated with the Muc phenotype.

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

von Wright A

Research and Development Department, Valio Finnish Co-operative Dairies' Association, SF-00181 Helsinki 18, Finland.

Tynkkynen S

References (8)

8 references, click to expand

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Article Info

Journal

Applied and environmental microbiology

Abbr.

Appl Environ Microbiol

ISSN

0099-2240

DOI

10.1128/aem.53.6.1385-1386.1987

Published

1987-06-00

Pages

1385-6

Language

English

Region

United States

NLM ID

7605801

PMCID

PMC203875

External Links

PubMed source DOI: 10.1128/aem.53.6.1385-1386.1987 PMC Full Text View journal details

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Construction of Streptococcus lactis subsp. lactis Strains with a Single Plasmid Associated with Mucoid Phenotype.

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