Production and Regeneration of Lactobacillus casei Protoplasts.
Methods for the production and regeneration of Lactobacillus casei protoplasts are described. Protoplasts of L. casei strains were obtained by treatment with mutanolysin or with mutanolysin and lysozyme together in a protoplast formation buffer containing 0.02 M HEPES (N-2-hydroxyethylpiperazine-N'-2-ethanesulfonic acid) (pH 7.0), 1 mM MgCl(2), 0.5% gelatin, and 0.3 M raffinose. Cells were regenerated on a complex medium supplemented with bovine serum albumin, MgCl(2), CaCl(2), gelatin, and raffinose. Lengthy digestion with lytic enzymes inhibited the capacity of protoplasts to regenerate. The optimum conditions of protoplast formation varied from strain to strain. Using predetermined optimal conditions it was possible to prepare protoplasts of several L. casei strains and regenerate them with 10 to 40% efficiency. The methods were applicable to other species of lactobacilli as well.
31 references, click to expand
-
Purification and properties of a membrane-associated, folate-binding protein from Lactobacillus casei.J Biol Chem. 1977 Jun 10;252(11):3760-5 PMID: 16917
-
High frequency transformation of Bacillus subtilis protoplasts by plasmid DNA.Mol Gen Genet. 1979 Jan 5;168(1):111-5 PMID: 107388
-
Transformation of plasmid DNA into Streptomyces at high frequency.Nature. 1978 Jul 27;274(5669):398-400 PMID: 672966
-
Fusion of protoplasts of Bacillus megaterium.Proc Natl Acad Sci U S A. 1976 Jun;73(6):2147-50 PMID: 819933
-
Transformation of Streptococcus sanguis Challis by plasmid deoxyribonucleic acid from Streptococcus faecalis.J Bacteriol. 1976 Oct;128(1):347-55 PMID: 824275
-
Bactoprenol, ATPase and acetate activating enzymes of a vesicular fraction from Lactobacillus casei.Eur J Biochem. 1969 Dec;11(3):582-91 PMID: 4244061
-
A low-viscosity epoxy resin embedding medium for electron microscopy.J Ultrastruct Res. 1969 Jan;26(1):31-43 PMID: 4887011
-
Development of competence in the Bacillus subtilis transformation system.J Bacteriol. 1967 Sep;94(3):562-70 PMID: 4962301
-
Gelatin-induced reversion of protoplasts of Bacillus subtilis to the bacillary form: electron-microscopic and physical study.J Bacteriol. 1968 Dec;96(6):2154-70 PMID: 4972919
-
Gelatin-induced reversion of protoplasts of Bacillus subtilis to the bacillary form: biosynthesis of macromolecules and wall during successive steps.J Bacteriol. 1969 Aug;99(2):576-89 PMID: 4980068
-
Reversion of Bacillus subtilis protoplasts to the bacillary form induced by exogenous cell wall, bacteria and by growth in membrane filters.J Gen Microbiol. 1970 May;61(2):233-43 PMID: 4991004
-
Molecular cloning of the gene for dihydrofolate reductase from Lactobacillus casei.Gene. 1982 Feb;17(2):229-33 PMID: 6282715
-
Helper plasmid cloning in Streptococcus sanguis: cloning of a tetracycline resistance determinant from the Streptococcus mutans chromosome.J Bacteriol. 1982 Oct;152(1):215-22 PMID: 6288658
-
Cloning and expression of the beta-D-phosphogalactoside galactohydrolase gene of Lactobacillus casei in Escherichia coli K-12.J Bacteriol. 1982 Dec;152(3):1138-46 PMID: 6292163
-
Transformation of intact yeast cells treated with alkali cations.J Bacteriol. 1983 Jan;153(1):163-8 PMID: 6336730
-
Genetic transformation of Bacillus brevis 47, a protein-secreting bacterium, by plasmid DNA.J Bacteriol. 1983 Dec;156(3):1130-4 PMID: 6358189
-
Biological nitrogen fixation: primary structure of the Klebsiella pneumoniae nifH and nifD genes.J Mol Appl Genet. 1981;1(1):71-81 PMID: 6809876
-
[Protoplast formation of Lactobacillus casei induced by endo-N-acetylmuramidase].Nihon Saikingaku Zasshi. 1982 Jul;37(4):777-85 PMID: 6816954
-
The microbiological production of food and drink.Sci Am. 1981 Sep;245(3):126-34, 136, 138 PMID: 6895124
-
Monomer plasmid DNA transforms Streptococcus pneumoniae.Mol Gen Genet. 1981;181(1):57-62 PMID: 6938758
-
Method for the lysis of Gram-positive, asporogenous bacteria with lysozyme.Appl Environ Microbiol. 1980 Jan;39(1):153-8 PMID: 6986847
-
Plasmid transfer and genetic recombination by protoplast fusion in staphylococci.J Bacteriol. 1981 Jan;145(1):74-81 PMID: 7007333
-
Properties and transforming activities of two plasmids in Streptococcus pneumoniae.Mol Gen Genet. 1980;180(3):573-8 PMID: 7007819
-
Mutanolysin-induced spheroplasts of Streptococcus mutants are true protoplasts.Infect Immun. 1981 Feb;31(2):808-15 PMID: 7012022
-
Protoplast fusion in Streptomyces: conditions for efficient genetic recombination and cell regeneration.J Gen Microbiol. 1981 Nov;127(1):137-46 PMID: 7338732
-
An antigenic analysis of Lactobacillus acidophilus.J Infect Dis. 1962 May-Jun;110:258-67 PMID: 13889379
-
The use of lead citrate at high pH as an electron-opaque stain in electron microscopy.J Cell Biol. 1963 Apr;17:208-12 PMID: 13986422
-
ENZYMICALLY AND PHYSICALLY INDUCED INHERITANCE CHANGES IN BACILLUS SUBTILIS.J Mol Biol. 1963 Dec;7:721-38 PMID: 14102204
-
Mutanolysin, bacteriolytic agent for cariogenic Streptococci: partial purification and properties.Antimicrob Agents Chemother. 1974 Aug;6(2):156-65 PMID: 15828186
-
Clostridium acetobutylicum Protoplast Formation and Regeneration.Appl Environ Microbiol. 1982 Mar;43(3):719-21 PMID: 16345980
-
Transformation of Streptococcus lactis Protoplasts by Plasmid DNA.Appl Environ Microbiol. 1982 May;43(5):1213-5 PMID: 16346019
Contact
No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong
Qilu Normal University · Genelibs Bioinformatics Lab
750 Shunhua Rd, Jinan
2F, Bldg F, University Science Park
Tel: 0531-88819269
WeChat Official Account
Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.
Business Email
E-mail: product@genelibs.com
Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2
