Home LiteratureArticle Details
PMID: 16345746 Published · ppublish English Journal Article

Detection of rotavirus in sewage.

Applied and environmental microbiology ·Vol. 41 ·No. 4 ·1981-04-00 ·Pages 1043-5

Steinmann J

Abstract

FOR DETECTION OF ROTAVIRUS, DOMESTIC SEWAGE WAS CONCENTRATED BY TWO DIFFERENT METHODS: (i) adsorption to and elution from positively charged Seitz filters, followed by ultracentrifugation, and (ii) chemical precipitation. The concentrated fluids were tested by an enzyme-linked immunosorbent assay and electron microscopy. In 6 of 24 (25%) samples, rotavirus was detectable after the combined filtration and ultracentrifugation technique with both an enzyme-linked immunosorbent assay and electron microscopy. No positive results were obtained after chemical precipitation.

Authors & Affiliations
1 authors, click to expand affiliations / ORCID
Steinmann J
Department of Medical Microbiology, University of Kiel, 2300 Kiel 1, West Germany.
References (15)
15 references, click to expand
  1. Rotavirus stability and inactivation.
    J Gen Virol. 1979 May;43(2):403-9 PMID: 39115
  2. Concentration of seeded simian rotavirus SA-11 from potable waters by using talc-celite layers and hydroextraction.
    Appl Environ Microbiol. 1980 Mar;39(3):493-9 PMID: 6247971
  3. Enzyme-linked immunosorbent assay (ELISA) for detection of human reovirus-like agent of infantile gastroenteritis.
    Lancet. 1977 Aug 6;2(8032):263-7 PMID: 69877
  4. Epidemic acute diarrhoea in adults associated with infantile gastroenteritis virus.
    Lancet. 1978 Nov 11;2(8098):1056-7 PMID: 82077
  5. A waterborne outbreak of gastroenteritis with secondary person-to-person spread. Association with a viral agent.
    Lancet. 1979 May 5;1(8123):964-6 PMID: 87627
  6. Human reovirus-like agent as the major pathogen associated with "winter" gastroenteritis in hospitalized infants and young children.
    N Engl J Med. 1976 Apr 29;294(18):965-72 PMID: 176586
  7. Routine diagnosis of human rotaviruses in stools.
    J Clin Pathol. 1978 Feb;31(2):175-8 PMID: 204665
  8. Comparison between adsorption of poliovirus and rotavirus by aluminum hydroxide and activated sludge flocs.
    Appl Environ Microbiol. 1978 Feb;35(2):360-3 PMID: 205173
  9. Fatal rotavirus gastroenteritis: an analysis of 21 cases.
    Am J Dis Child. 1978 May;132(5):477-9 PMID: 206132
  10. Rotavirus infections in adults in association with acute gastroenteritis.
    J Med Virol. 1978;2(1):21-8 PMID: 210251
  11. A simple immunofluorescent technique for the detection of human rotavirus.
    J Med Virol. 1979;3(3):189-94 PMID: 225439
  12. A microtiter solid phase radioimmunoassay for detection of the human reovirus-like agent in stools.
    J Immunol. 1977 Apr;118(4):1275-9 PMID: 321688
  13. Rapid detection of Australia-SH antigen and antibody by a simple and sensitive technique of immunoelectronmicroscopy.
    Can J Microbiol. 1971 Jul;17(7):993-1000 PMID: 4106186
  14. Stability of simian rotavirus in fresh and estuarine water.
    Appl Environ Microbiol. 1980 Jan;39(1):1-5 PMID: 6243899
  15. Letter: Probable in-vitro cultivation of human reovirus-like agent of infantile diarroea.
    Lancet. 1976 Jan 10;1(7950):98-9 PMID: 54627
Article Info
Journal
Applied and environmental microbiology
Abbr.
Appl Environ Microbiol
ISSN
0099-2240
Published
1981-04-00
Pages
1043-5
Language
English
Region
United States
NLM ID
7605801
PMCID
PMC243854
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com