Modified Fluorescent Technique, Using Rhodamine, for Studies of Rhizobium japonicum-Soybean Symbiosis.

Applied and environmental microbiology ·Vol. 37 ·No. 6 ·1979-06-00 ·Pages 1243-4

Hughes TA, Lecce JG, Elkan GH

Abstract

Rhodamine-conjugated capsular polysaccharides isolated from Rhizobium japonicum 61A76NS were used to examine binding between the labeled polysaccharides and soybean roots. Fluorescein-labeled polysaccharides were not satisfactory because soybean root hairs autofluoresce in the fluorescein region.

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Hughes T A

Department of Microbiology, North Carolina State University, Raleigh, North Carolina 27650.

Lecce J G

Elkan G H

References (3)

3 references, click to expand

Rhizobium japonicum derivatives differing in nitrogen-fixing efficiency and carbohydrate utilization.
Appl Environ Microbiol. 1976 Oct;32(4):511-9 PMID: 988784
Lectins: a possible basis for specificity in the Rhizobium--legume root nodule symbiosis.
Science. 1974 Jul 19;185(4147):269-71 PMID: 17812054
Receptor site on clover and alfalfa roots for Rhizobium.
Appl Environ Microbiol. 1977 Jan;33(1):132-6 PMID: 16345183

Article Info

Journal

Applied and environmental microbiology

Abbr.

Appl Environ Microbiol

ISSN

0099-2240

DOI

10.1128/aem.37.6.1243-1244.1979

Published

1979-06-00

Pages

1243-4

Language

English

Region

United States

NLM ID

7605801

PMCID

PMC243386

External Links

PubMed source DOI: 10.1128/aem.37.6.1243-1244.1979 PMC Full Text View journal details

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Modified Fluorescent Technique, Using Rhodamine, for Studies of Rhizobium japonicum-Soybean Symbiosis.

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