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PMID: 1630901 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Systematic sequencing of the Escherichia coli genome: analysis of the 0-2.4 min region.

Nucleic acids research ·Vol. 20 ·No. 13 ·1992-07-11 ·Pages 3305-8

Yura T, Mori H, Nagai H, Nagata T, Ishihama A, Fujita N, Isono K, Mizobuchi K, Nakata A

Abstract

A contiguous 111,402-nucleotide sequence corresponding to the 0 to 2.4 min region of the E. coli chromosome was determined as a first step to complete structural analysis of the genome. The resulting sequence was used to predict open reading frames and to search for sequence similarity against the PIR protein database. A number of novel genes were found whose predicted protein sequences showed significant homology with known proteins from various organisms, including several clusters of genes similar to those involved in fatty acid metabolism in bacteria (e.g., betT, baiF) and higher organisms, iron transport (sfuA, B, C) in Serratia marcescens, and symbiotic nitrogen fixation or electron transport (fixA, B, C, X) in Azorhizobium caulinodans. In addition, several genes and IS elements that had been mapped but not sequenced (e.g., leuA, B, C, D) were identified. We estimate that about 90 genes are represented in this region of the chromosome with little spacer.

MeSH Terms
Bacterial Proteins/genetics Escherichia coli/genetics Genes, Bacterial/genetics Genome, Bacterial Molecular Sequence Data Multigene Family/genetics Rhizobiaceae/genetics Serratia marcescens/genetics
Chemicals
Bacterial Proteins
Authors & Affiliations
9 authors, click to expand affiliations / ORCID
Yura T
Institute for Virus Research, Kyoto University, Japan.
Mori H
Nagai H
Nagata T
Ishihama A
Fujita N
Isono K
Mizobuchi K
Nakata A
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18 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/20.13.3305
Published
1992-07-11
Pages
3305-8
Language
English
Region
England
NLM ID
0411011
PMCID
PMC312481
Subset
IM
Grants
PHS HHS · DDBJ/D10483 · United States
External Links
PubMed source DOI: 10.1093/nar/20.13.3305 PMC Full Text View journal details
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