Minimal growth requirements for Clostridium perfringens and isolation of auxotrophic mutants.

Applied microbiology ·Vol. 29 ·No. 1 ·1975-01-00 ·Pages 1-6

Sebald M, Costilow RN

Abstract

The minimal growth requirements for two strains of Clostridium perfringens were defined, and both synthetic and semisynthetic plating media were developed. Plate counts of the wild-type strains on both of these minimal media were equivalent to those on complex media. A number of auxotrophic mutants of each strain were isolated, and their phenotypes were defined.

MeSH Terms

Adenine/metabolism Amino Acids/metabolism Anaerobiosis Clostridium perfringens/growth & development,isolation & purification,metabolism Culture Media Lactose/metabolism Lysine/metabolism Mutagens Mutation Nicotinic Acids/metabolism Nitrosoguanidines Phenotype Proline/metabolism Salts/metabolism Thiamine/metabolism Thioglycolates Uracil/metabolism Vitamins/metabolism

Chemicals

Amino Acids Culture Media Mutagens Nicotinic Acids Nitrosoguanidines Salts Thioglycolates Vitamins Uracil Proline Lactose Adenine Lysine Thiamine

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Sebald M

Costilow R N

References (9)

9 references, click to expand

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Ann Inst Pasteur (Paris). 1972 Jun;122(6):1117-27 PMID: 4342633
Sporulation and enterotoxin production by mutants of Clostridium perfringens.
J Bacteriol. 1972 Apr;110(1):378-91 PMID: 4336110
Chemically defined medium for the growth of Clostridium perfringens.
Appl Microbiol. 1971 Oct;22(4):738-9 PMID: 4331776
A temperate bacteriophage of Clostridium perfringens.
Can J Microbiol. 1968 Oct;14(10):1085-93 PMID: 4176099
Chemically defined medium for growth and sporulation of Clostridium perfringens.
Appl Microbiol. 1972 Nov;24(5):755-9 PMID: 4344959
The nutritional requirements of Clostridium perfringens.
J Gen Microbiol. 1957 Apr;16(2):317-29 PMID: 13416509
Production of alpha toxin of Clostridium perfringens. I. Preparation of the reproducible peptone medium for the production of the toxin of high potency.
Jpn J Med Sci Biol. 1956 Jun;9(3):81-91 PMID: 13376194
Intracellular development of a bacteriophage of Clostridium perfringens.
Can J Microbiol. 1970 Oct;16(10):983-8 PMID: 5483649
The bacteriophages of Clostridium perfringens.
J Gen Microbiol. 1959 Dec;21:622-30 PMID: 13831994

Article Info

Journal

Applied microbiology

Abbr.

Appl Microbiol

ISSN

0003-6919

DOI

10.1128/am.29.1.1-6.1975

Published

1975-01-00

Pages

1-6

Language

English

Region

United States

NLM ID

7605802

PMCID

PMC186899

Subset

External Links

PubMed source DOI: 10.1128/am.29.1.1-6.1975 PMC Full Text

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Minimal growth requirements for Clostridium perfringens and isolation of auxotrophic mutants.

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