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PMID: 1629323 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Pulsed-field gel electrophoresis of NotI digests of leptospiral DNA: a new rapid method of serovar identification.

Journal of clinical microbiology ·Vol. 30 ·No. 7 ·1992-07-00 ·Pages 1696-702

Herrmann JL, Bellenger E, Perolat P, Baranton G, Saint Girons I

Abstract

Fingerprints for 72 reference serovar strains of pathogenic Leptospira spp. were obtained by pulsed-field gel electrophoresis (PFGE) following NotI restriction digests of the chromosome. These strains included the serovar reference strains of serogroups Australis, Ballum, Bataviae, Grippotyphosa, Panama, Pomona, and Pyrogenes. Sixty-four serovars could be identified by a unique NotI restriction profile. The remaining serovars were differentiated by chromosomal digestion with SgrAI. These included four serovars from serogroup Australis, two serovars from serogroup Ballum, and two serovars from serogroup Bataviae. Thirteen of 18 recent clinical isolates identified by microagglutination test and cross-adsorption procedure were correctly typed by PFGE. The results indicate that PFGE, which is considerably more rapid than serology, should be useful for identification and epidemiological studies.

MeSH Terms
Animals Bacterial Typing Techniques DNA Fingerprinting DNA, Bacterial/genetics Deoxyribonucleases, Type II Site-Specific Electrophoresis, Gel, Pulsed-Field/methods Humans Leptospira/classification,genetics,isolation & purification
Chemicals
DNA, Bacterial endodeoxyribonuclease SgrAI Deoxyribonucleases, Type II Site-Specific GCGGCCGC-specific type II deoxyribonucleases
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Herrmann J L
Unité de Bactériologie Moléculaire et Médicale, Institut Pasteur, Paris, France.
Bellenger E
Perolat P
Baranton G
Saint Girons I
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Article Info
Journal
Journal of clinical microbiology
Abbr.
J Clin Microbiol
ISSN
0095-1137
DOI
10.1128/jcm.30.7.1696-1702.1992
Published
1992-07-00
Pages
1696-702
Language
English
Region
United States
NLM ID
7505564
PMCID
PMC265366
Subset
IM
External Links
PubMed source DOI: 10.1128/jcm.30.7.1696-1702.1992 PMC Full Text View journal details
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