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PMID: 16278649 Published · ppublish English Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Compensatory ingestion upon dietary restriction in Drosophila melanogaster.

Nature methods ·Vol. 2 ·No. 11 ·2005-11-00 ·Pages 813-5

Carvalho GB, Kapahi P, Benzer S

Abstract

Dietary restriction extends the lifespan of numerous, evolutionarily diverse species. In D. melanogaster, a prominent model for research on the interaction between nutrition and longevity, dietary restriction is typically based on medium dilution, with possible compensatory ingestion commonly being neglected. Possible problems with this approach are revealed by using a method for direct monitoring of D. melanogaster feeding behavior. This demonstrates that dietary restriction elicits robust compensatory changes in food consumption. As a result, the effect of medium dilution is overestimated and, in certain cases, even fully compensated for. Our results strongly indicate that feeding behavior and nutritional composition act concertedly to determine fly lifespan. Feeding behavior thus emerges as a central element in D. melanogaster aging.

MeSH Terms
Aging/physiology Animal Feed Animals Drosophila melanogaster/physiology Feeding Behavior/physiology Food Deprivation/physiology Longevity/physiology Nutritional Physiological Phenomena/physiology Reproducibility of Results
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Carvalho Gil B
Division of Biology 156-29, California Institute of Technology, Pasadena, California 91125, USA.
Kapahi Pankaj
Benzer Seymour
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Article Info
Journal
Nature methods
Abbr.
Nat Methods
ISSN
1548-7091
DOI
10.1038/nmeth798
Published
2005-11-00
Pages
813-5
Language
English
Region
United States
NLM ID
101215604
PMCID
PMC2745347
Subset
IM
Grants
NIA NIH HHS · R21 AG028241-02 · United States
NIDDK NIH HHS · DK070154 · United States
NIA NIH HHS · AG24366 · United States
NIA NIH HHS · R21 AG028241 · United States
NIA NIH HHS · R01 AG024366-01 · United States
NIA NIH HHS · R01 AG024366-02 · United States
Medical Research Council · G9900989 · United Kingdom
NIA NIH HHS · RL1 AG032113 · United States
NIA NIH HHS · R21 AG028241-01 · United States
NIA NIH HHS · R01 AG024366-03 · United States
NIA NIH HHS · R01 AG024366-02S1 · United States
NIA NIH HHS · AG16630 · United States
Corrections
CommentIn
PMID: 18309307
External Links
PubMed source DOI: 10.1038/nmeth798 PMC Full Text View journal details
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