Quantitative trait loci mapped to single-nucleotide resolution in yeast.

Nature genetics ·Vol. 37 ·No. 12 ·2005-12-00 ·Pages 1333-40

Deutschbauer AM, Davis RW

Abstract

Identifying the genetic variation underlying quantitative trait loci remains problematic. Consequently, our molecular understanding of genetically complex, quantitative traits is limited. To address this issue directly, we mapped three quantitative trait loci that control yeast sporulation efficiency to single-nucleotide resolution in a noncoding regulatory region (RME1) and to two missense mutations (TAO3 and MKT1). For each quantitative trait locus, the responsible polymorphism is rare among a diverse set of 13 yeast strains, suggestive of genetic heterogeneity in the control of yeast sporulation. Additionally, under optimal conditions, we reconstituted approximately 92% of the sporulation efficiency difference between the two genetically distinct parents by engineering three nucleotide changes in the appropriate yeast genome. Our results provide the highest resolution to date of the molecular basis of a quantitative trait, showing that the interaction of a few genetic variants can have a profound phenotypic effect.

MeSH Terms

Adaptor Proteins, Signal Transducing Base Sequence Carrier Proteins/genetics Chromosome Mapping Epistasis, Genetic Genes, Fungal Molecular Sequence Data Mutation, Missense Nucleotides/genetics Quantitative Trait Loci Repressor Proteins/genetics Saccharomyces cerevisiae/genetics,physiology Saccharomyces cerevisiae Proteins/genetics Spores, Fungal/genetics,growth & development

Chemicals

Adaptor Proteins, Signal Transducing Carrier Proteins MKT1 protein, S cerevisiae Nucleotides RME1 protein, S cerevisiae Repressor Proteins Saccharomyces cerevisiae Proteins TAO3 protein, S cerevisiae

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Deutschbauer Adam M

Department of Biochemistry, Stanford University School of Medicine, Stanford, California 94305, USA. AMDeutschbauer@lbl.gov

Davis Ronald W

Article Info

Journal

Nature genetics

Abbr.

Nat Genet

ISSN

1061-4036

DOI

10.1038/ng1674

amdeutschbauer@lbl.gov

Published

2005-12-00

Epub

2005-00-06

Pages

1333-40

Language

English

Region

United States

NLM ID

9216904

Subset

Databases

GENBANK

DQ115389, DQ115390, DQ115391, DQ115392, DQ115393, DQ116799, DQ116800, DQ116801, DQ116802, DQ116803, DQ116804, DQ116805, DQ116806, DQ116807, DQ116808, DQ116809, DQ116810, DQ116811, DQ116812, DQ116813, DQ116814, DQ116815, DQ116816, DQ116817, DQ116818, DQ116819, DQ116820, DQ116821, DQ116822, DQ116823, DQ116824, DQ116825, DQ116826, DQ116827, DQ116828

External Links

PubMed source DOI: 10.1038/ng1674 View journal details

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Quantitative trait loci mapped to single-nucleotide resolution in yeast.

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