A 5'-nuclease real-time reverse transcriptase-polymerase chain reaction assay for the detection of a broad range of influenza A subtypes, including H5N1.

Diagnostic microbiology and infectious disease ·Vol. 53 ·No. 4 ·2005-12-00 ·Pages 335-7

Whiley DM, Sloots TP

Abstract

A 5'-nuclease real-time reverse transcriptase-polymerase chain reaction assay was developed for the detection of influenza type A and was validated using a range of influenza A subtypes, including avian strains, and 126 nasopharyngeal aspirate samples. The results show the assay is suitable for screening for influenza A infections, particularly in regions where avian strains may be circulating.

MeSH Terms

Animals Birds/virology Humans Influenza A Virus, H5N1 Subtype/classification,genetics,isolation & purification Influenza A virus/classification,genetics,isolation & purification Influenza in Birds/virology Influenza, Human/virology Nasopharynx/virology Reverse Transcriptase Polymerase Chain Reaction/methods

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Whiley David M

Clinical Virology Research Unit, Sir Albert Sakzewski Virus Research Centre, Royal Children's Hospital and Health Service District, Queensland, 4029 Australia. d.whiley@uq.edu.au

Sloots Theo P

Article Info

Journal

Diagnostic microbiology and infectious disease

Abbr.

Diagn Microbiol Infect Dis

ISSN

0732-8893

DOI

10.1016/j.diagmicrobio.2005.08.002

d.whiley@uq.edu.au

Published

2005-12-00

Epub

2005-00-02

Pages

335-7

Language

English

Region

United States

NLM ID

8305899

Subset

External Links

PubMed source DOI: 10.1016/j.diagmicrobio.2005.08.002 View journal details

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A 5'-nuclease real-time reverse transcriptase-polymerase chain reaction assay for the detection of a broad range of influenza A subtypes, including H5N1.

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