Home LiteratureArticle Details
PMID: 16258851 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Effect of general anesthetics on amyloid precursor protein and mRNA levels in the rat brain.

Neurochemical research ·Vol. 30 ·No. 8 ·2005-08-00 ·Pages 1021-6

Palotás M, Palotás A, Bjelik A, Pákáski M, Hugyecz M, Janka Z, Kálmán J

Abstract

The incidence of Alzheimer's disease is elevated after exposure to surgical interventions. Since amyloid precursor protein (APP) and its neurotoxic derivatives play key roles in the development of Alzheimer dementia, the role of general anesthesia is controversial in the development of cognitive decline. As such, the effect of anesthetics on APP protein and mRNA levels was assessed utilizing semiquantitative Western-immunoblot and reverse transcription polymerase chain reaction (RT-PCR) in brains of rats following intraperitoneal treatment with propofol and thiopental. The anesthetics did not change cortical APP protein and mRNA concentration considerably. These results indicate that both propofol and thiopental are considered to be relatively safe with respect to APP metabolism.

MeSH Terms
Amyloid beta-Protein Precursor/genetics,metabolism Anesthetics, General/pharmacology Animals Base Sequence Brain/drug effects,metabolism DNA Primers Male RNA, Messenger/genetics,metabolism Rats Rats, Sprague-Dawley Reverse Transcriptase Polymerase Chain Reaction
Chemicals
Amyloid beta-Protein Precursor Anesthetics, General DNA Primers RNA, Messenger
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Palotás Miklós
Department of Psychiatry, Albert Szent-Györgyi Medical and Pharmaceutical Center, Faculty of Medicine, University of Szeged, H-6721, Szeged, Semmelweis u. 6, Hungary.
Palotás András
Bjelik Annamária
Pákáski Magdolna
Hugyecz Marietta
Janka Zoltán
Kálmán János
References (22)
22 references, click to expand
  1. Amyloid precursor protein accumulates in regions of neurodegeneration following focal cerebral ischemia in the rat.
    Brain Res. 1992 Oct 9;593(1):128-35 PMID: 1458315
  2. Isolation of low-molecular-weight proteins from amyloid plaque fibers in Alzheimer's disease.
    J Neurochem. 1986 Jun;46(6):1820-34 PMID: 3517233
  3. Microembolic brain injuries from cardiac surgery: are they seeds of future Alzheimer's disease?
    Ann N Y Acad Sci. 1997 Sep 26;826:386-9 PMID: 9329709
  4. A linear Lowry--Folin assay for both water-soluble and sodium dodecyl sulfate-solubilized proteins.
    Anal Biochem. 1978 Mar;85(1):295-300 PMID: 629390
  5. Amyloid precursor proteins protect neurons of transgenic mice against acute and chronic excitotoxic injuries in vivo.
    Neuroscience. 1997 May;78(1):135-46 PMID: 9135095
  6. Membrane-associated forms of the beta A4 amyloid protein precursor of Alzheimer's disease in human platelet and brain: surface expression on the activated human platelet.
    Blood. 1994 Jul 1;84(1):133-42 PMID: 8018913
  7. Effect of open heart surgery on intellectual function.
    Scand J Thorac Cardiovasc Surg Suppl. 1974;(15):1-63 PMID: 4533364
  8. Medical history and the risk of Alzheimer's disease: a collaborative re-analysis of case-control studies. EURODEM Risk Factors Research Group.
    Int J Epidemiol. 1991;20 Suppl 2:S36-42 PMID: 1833352
  9. Risk of Alzheimer's disease and duration of NSAID use.
    Neurology. 1997 Mar;48(3):626-32 PMID: 9065537
  10. Alzheimer's disease and cumulative exposure to anesthesia: a case-control study.
    J Am Geriatr Soc. 1994 Feb;42(2):198-201 PMID: 8126336
  11. The effects of propofol in the area postrema of rats.
    Anesth Analg. 2001 Apr;92(4):934-42 PMID: 11273930
  12. A case-control study on Alzheimer's disease and exposure to anesthesia.
    Neurol Sci. 2002 Apr;23(1):11-4 PMID: 12111615
  13. Hypoglycemia enhances the expression of mRNA encoding beta-amyloid precursor protein in rat primary cortical astroglial cells.
    Brain Res. 1997 Oct 24;772(1-2):247-51 PMID: 9406980
  14. Markers of axonal injury in post mortem human brain.
    Acta Neuropathol. 1994;88(5):433-9 PMID: 7847072
  15. Widespread inhibition of sodium channel-dependent glutamate release from isolated nerve terminals by isoflurane and propofol.
    Anesthesiology. 2001 Dec;95(6):1460-6 PMID: 11748406
  16. Alpha-2 adrenoceptor activity affects propofol-induced sleep time.
    Anesth Analg. 2002 May;94(5):1201-6, table of contents PMID: 11973190
  17. Effects of surgery on the mental status of older persons. A meta-analytic review.
    J Geriatr Psychiatry Neurol. 1990 Oct-Dec;3(4):184-91 PMID: 2149929
  18. Anesthetic considerations in patients with Alzheimer's disease.
    J Clin Anesth. 2003 Feb;15(1):52-8 PMID: 12657410
  19. Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction.
    Anal Biochem. 1987 Apr;162(1):156-9 PMID: 2440339
  20. Cellular actions of beta-amyloid precursor protein and its soluble and fibrillogenic derivatives.
    Physiol Rev. 1997 Oct;77(4):1081-132 PMID: 9354812
  21. Cognitive effects after epidural vs general anesthesia in older adults. A randomized trial.
    JAMA. 1995 Jul 5;274(1):44-50 PMID: 7791257
  22. Postoperative delirium in the elderly.
    Anesth Analg. 1995 Jun;80(6):1223-32 PMID: 7762856
Article Info
Journal
Neurochemical research
Abbr.
Neurochem Res
ISSN
0364-3190
DOI
10.1007/s11064-005-6786-7
Published
2005-08-00
Pages
1021-6
Language
English
Region
United States
NLM ID
7613461
Subset
IM
External Links
PubMed source DOI: 10.1007/s11064-005-6786-7 View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2