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PMID: 16254380 Published · ppublish English Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Retroviral restriction factor TRIM5alpha is a trimer.

Journal of virology ·Vol. 79 ·No. 22 ·2005-11-00 ·Pages 14446-50

Mische CC, Javanbakht H, Song B, Diaz-Griffero F, Stremlau M, Strack B, Si Z, Sodroski J

Abstract

The retrovirus restriction factor TRIM5alpha targets the viral capsid soon after entry. Here we show that the TRIM5alpha protein oligomerizes into trimers. The TRIM5alpha coiled-coil and B30.2(SPRY) domains make important contributions to the formation and/or stability of the trimers. A functionally defective TRIM5alpha mutant with the RING and B-box 2 domains deleted can form heterotrimers with wild-type TRIM5alpha, accounting for the observed dominant-negative activity of the mutant protein. Trimerization potentially allows TRIM5alpha to interact with threefold pseudosymmetrical structures on retroviral capsids.

MeSH Terms
Animals Binding Sites Capsid/metabolism Capsid Proteins/metabolism Cell Line Chlorocebus aethiops Genetic Variation HeLa Cells Humans Macaca mulatta Proteins/metabolism Retroviridae/metabolism Transfection Ubiquitin-Protein Ligases
Chemicals
Capsid Proteins Proteins TRIM5(alpha) protein, rhesus monkey Ubiquitin-Protein Ligases
Authors & Affiliations
8 authors, click to expand affiliations / ORCID
Mische Claudia C
Department of Cancer Immunology and AIDS, Dana-Farber Cancer Institute, Division of AIDS, Harvard Medical School, Boston, Massachusetts 02115, USA.
Javanbakht Hassan
Song Byeongwoon
Diaz-Griffero Felipe
Stremlau Matthew
Strack Bettina
Si Zhihai
Sodroski Joseph
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.79.22.14446-14450.2005
Published
2005-11-00
Pages
14446-50
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC1280198
Subset
IM
Grants
NIAID NIH HHS · R01 AI063987 · United States
NIAID NIH HHS · P30 AI028691 · United States
NHLBI NIH HHS · HL54785 · United States
NHLBI NIH HHS · P50 HL054785 · United States
NIAID NIH HHS · AI28691 · United States
NIAID NIH HHS · AI063987 · United States
External Links
PubMed source DOI: 10.1128/JVI.79.22.14446-14450.2005 PMC Full Text View journal details
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