Home LiteratureArticle Details
PMID: 1624449 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.

Genetic analysis of absB, a Streptomyces coelicolor locus involved in global antibiotic regulation.

Journal of bacteriology ·Vol. 174 ·No. 14 ·1992-07-00 ·Pages 4622-8

Adamidis T, Champness W

Abstract

The filamentous soil bacterium Streptomyces coelicolor is known to produce four antibiotics which are genetically and structurally distinct. An extensive search for antibiotic regulatory mutants led to the discovery of absB mutants, which are antibiotic deficient but sporulation proficient. Genetic analysis of the absB mutants has resulted in definition of the absB locus at 5 o'clock on the genetic map. Multiple cloned copies of the actII-ORF4 gene, an activator of synthesis of the antibiotic actinorhodin, restore actinorhodin biosynthetic capability to the absB mutants. These results are interpreted to mean that the failure of absB mutants to produce antibiotics results from decreased expression of the antibiotic genes. The absB gene is proposed to be involved in global regulation of antibiotic synthesis.

Related Genes
absB
MeSH Terms
Anthraquinones/metabolism Anti-Bacterial Agents/biosynthesis Crosses, Genetic Gene Expression Regulation, Bacterial Genes, Regulator/genetics Multigene Family/genetics Mutagenesis Mutation/genetics Streptomyces/genetics,metabolism
Chemicals
Anthraquinones Anti-Bacterial Agents actinorhodin
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Adamidis T
Genetics Program, Michigan State University, East Lansing 48824-1101.
Champness W
References (31)
31 references, click to expand
  1. Mutations in a new Streptomyces coelicolor locus which globally block antibiotic biosynthesis but not sporulation.
    J Bacteriol. 1990 Jun;172(6):2962-9 PMID: 2345130
  2. New loci required for Streptomyces coelicolor morphological and physiological differentiation.
    J Bacteriol. 1988 Mar;170(3):1168-74 PMID: 3343216
  3. Cloning and expression of the tyrosinase gene from Streptomyces antibioticus in Streptomyces lividans.
    J Gen Microbiol. 1983 Sep;129(9):2703-14 PMID: 6313861
  4. Mutational cloning in Streptomyces and the isolation of antibiotic production genes.
    Gene. 1983 Dec;26(1):67-78 PMID: 6323253
  5. Molecular cloning of the whole biosynthetic pathway of a Streptomyces antibiotic and its expression in a heterologous host.
    Nature. 1984 May 31-Jun 6;309(5967):462-4 PMID: 6328317
  6. The expression of Streptomyces and Escherichia coli drug-resistance determinants cloned into the Streptomyces phage phi C31.
    Gene. 1982 Jul-Aug;19(1):21-32 PMID: 6292047
  7. Genetic analysis of A-factor synthesis in Streptomyces coelicolor A3(2) and Streptomyces griseus.
    J Gen Microbiol. 1983 Sep;129(9):2939-44 PMID: 6415235
  8. CDA is a new chromosomally-determined antibiotic from Streptomyces coelicolor A3(2).
    J Gen Microbiol. 1983 Dec;129(12):3575-9 PMID: 6668466
  9. A pigmented mycelial antibiotic in Streptomyces coelicolor: control by a chromosomal gene cluster.
    J Gen Microbiol. 1980 Aug;119(2):333-40 PMID: 7229612
  10. A morphological and genetic mapping study of bald colony mutants of Streptomyces coelicolor.
    J Gen Microbiol. 1976 Oct;96(2):299-315 PMID: 186556
  11. Genetics of actinorhodin biosynthesis by Streptomyces coelicolor A3(2).
    J Gen Microbiol. 1979 Sep;114(1):35-43 PMID: 521794
  12. Genetic determination of methylenomycin synthesis by the SCP1 plasmid of Streptomyces coelicolor A3(2).
    J Gen Microbiol. 1977 Jan;98(1):239-52 PMID: 833570
  13. Characterization of temperate actinophage phi C31 isolated from Streptomyces coelicolor A3(2).
    J Virol. 1972 Feb;9(2):258-62 PMID: 5014931
  14. Genetic control of fertility in Streptomyces coelicolor A3(2): the IF fertility type.
    J Gen Microbiol. 1970 Nov;64(1):101-17 PMID: 5516602
  15. A new kind of fertility variant in Streptomyces coelicolor.
    Genetics. 1969 Jul;62(3):461-77 PMID: 5384479
  16. Tn4563 transposition in Streptomyces coelicolor and its application to isolation of new morphological mutants.
    J Bacteriol. 1991 Aug;173(16):5060-7 PMID: 1650343
  17. Phage vectors that allow monitoring of transcription of secondary metabolism genes in Streptomyces.
    Biotechnology (N Y). 1991 Jul;9(7):652-6 PMID: 1367661
  18. abaA, a new pleiotropic regulatory locus for antibiotic production in Streptomyces coelicolor.
    J Bacteriol. 1992 May;174(9):2958-67 PMID: 1569025
  19. TTA codons in some genes prevent their expression in a class of developmental, antibiotic-negative, Streptomyces mutants.
    Proc Natl Acad Sci U S A. 1991 Mar 15;88(6):2461-5 PMID: 1826053
  20. The act cluster contains regulatory and antibiotic export genes, direct targets for translational control by the bldA tRNA gene of Streptomyces.
    Cell. 1991 Aug 23;66(4):769-80 PMID: 1878971
  21. Extracellular complementation of a developmental mutation implicates a small sporulation protein in aerial mycelium formation by S. coelicolor.
    Cell. 1991 May 17;65(4):641-50 PMID: 2032288
  22. Transcriptional and translational features of a sporulation gene of Streptomyces griseus.
    Gene. 1990 Oct 30;95(1):57-63 PMID: 2123814
  23. Primary structure of AfsR, a global regulatory protein for secondary metabolite formation in Streptomyces coelicolor A3(2).
    Gene. 1990 Oct 30;95(1):49-56 PMID: 2253887
  24. The level of a transcript required for production of a Streptomyces coelicolor antibiotic is conditionally dependent on a tRNA gene.
    J Bacteriol. 1990 Nov;172(11):6189-93 PMID: 2228956
  25. Cloning and expression in a heterologous host of the complete set of genes for biosynthesis of the Streptomyces coelicolor antibiotic undecylprodigiosin.
    Gene. 1990 Sep 1;93(1):91-9 PMID: 2227430
  26. Physical and genetic characterisation of the gene cluster for the antibiotic actinorhodin in Streptomyces coelicolor A3(2).
    Mol Gen Genet. 1986 Oct;205(1):66-73 PMID: 3025560
  27. Genetic and biochemical characterization of the red gene cluster of Streptomyces coelicolor A3(2).
    J Gen Microbiol. 1985 Sep;131(9):2431-41 PMID: 2999302
  28. Nucleotide sequence, transcription and deduced function of a gene involved in polyketide antibiotic synthesis in Streptomyces coelicolor.
    Gene. 1988 Dec 30;74(2):305-20 PMID: 2469622
  29. Pleiotropic morphological and antibiotic deficiencies result from mutations in a gene encoding a tRNA-like product in Streptomyces coelicolor A3(2).
    Genes Dev. 1987 Dec;1(10):1305-10 PMID: 2448187
  30. xylE functions as an efficient reporter gene in Streptomyces spp.: use for the study of galP1, a catabolite-controlled promoter.
    J Bacteriol. 1989 Dec;171(12):6617-24 PMID: 2592344
  31. Nucleotide sequence and transcriptional analysis of the redD locus of Streptomyces coelicolor A3(2).
    J Bacteriol. 1990 Jan;172(1):326-33 PMID: 2294088
Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.174.14.4622-4628.1992
Published
1992-07-00
Pages
4622-8
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC206257
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.174.14.4622-4628.1992 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2