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PMID: 16210541 Published · ppublish English Journal Article Research Support, N.I.H., Extramural Research Support, U.S. Gov't, P.H.S.

Astrocytic purinergic signaling coordinates synaptic networks.

Science (New York, N.Y.) ·Vol. 310 ·No. 5745 ·2005-10-07 ·Pages 113-6

Pascual O, Casper KB, Kubera C, Zhang J, Revilla-Sanchez R, Sul JY, Takano H, Moss SJ, McCarthy K, Haydon PG

Abstract

To investigate the role of astrocytes in regulating synaptic transmission, we generated inducible transgenic mice that express a dominant-negative SNARE domain selectively in astrocytes to block the release of transmitters from these glial cells. By releasing adenosine triphosphate, which accumulates as adenosine, astrocytes tonically suppressed synaptic transmission, thereby enhancing the dynamic range for long-term potentiation and mediated activity-dependent, heterosynaptic depression. These results indicate that astrocytes are intricately linked in the regulation of synaptic strength and plasticity and provide a pathway for synaptic cross-talk.

MeSH Terms
Adenosine/metabolism Adenosine A1 Receptor Antagonists Adenosine Triphosphatases/antagonists & inhibitors Adenosine Triphosphate/analogs & derivatives,metabolism,pharmacology Animals Astrocytes/drug effects,physiology Cells, Cultured Excitatory Postsynaptic Potentials Hippocampus/drug effects,physiology In Vitro Techniques Long-Term Potentiation/drug effects Mice Mice, Transgenic Neuronal Plasticity/drug effects Purinergic P1 Receptor Antagonists Purinergic P2 Receptor Antagonists Receptor, Adenosine A1/metabolism Receptors, N-Methyl-D-Aspartate/antagonists & inhibitors,metabolism Receptors, Purinergic P1/metabolism Receptors, Purinergic P2/metabolism Synapses/physiology Synaptic Transmission/drug effects Xanthines/pharmacology
Chemicals
6-N,N-diethyl-beta,gamma-dibromomethylene-D-ATP Adenosine A1 Receptor Antagonists NR2B NMDA receptor Purinergic P1 Receptor Antagonists Purinergic P2 Receptor Antagonists Receptor, Adenosine A1 Receptors, N-Methyl-D-Aspartate Receptors, Purinergic P1 Receptors, Purinergic P2 Xanthines Adenosine Triphosphate 1,3-dipropyl-8-cyclopentylxanthine Adenosine Triphosphatases Adenosine
Authors & Affiliations
10 authors, click to expand affiliations / ORCID
Pascual Olivier
Department of Neuroscience, Conte Center for Integration at the Tripartite Synapse, University of Pennsylvania School of Medicine, Philadelphia, PA 19104, USA.
Casper Kristen B
Kubera Cathryn
Zhang Jing
Revilla-Sanchez Raquel
Sul Jai-Yoon
Takano Hajime
Moss Stephen J
McCarthy Ken
Haydon Philip G
Article Info
Journal
Science (New York, N.Y.)
Abbr.
Science
ISSN
1095-9203
Published
2005-10-07
Pages
113-6
Language
English
Region
United States
NLM ID
0404511
Subset
IM
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