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PMID: 16199614 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.

RNA interference identifies a calcium-dependent protein kinase involved in Medicago truncatula root development.

The Plant cell ·Vol. 17 ·No. 11 ·2005-11-00 ·Pages 2911-21

Ivashuta S, Liu J, Liu J, Lohar DP, Haridas S, Bucciarelli B, VandenBosch KA, Vance CP, Harrison MJ, Gantt JS

Abstract

Changes in cellular or subcellular Ca2+ concentrations play essential roles in plant development and in the responses of plants to their environment. However, the mechanisms through which Ca2+ acts, the downstream signaling components, as well as the relationships among the various Ca2+-dependent processes remain largely unknown. Using an RNA interference-based screen for gene function in Medicago truncatula, we identified a gene that is involved in root development. Silencing Ca2+-dependent protein kinase1 (CDPK1), which is predicted to encode a Ca2+-dependent protein kinase, resulted in significantly reduced root hair and root cell lengths. Inactivation of CDPK1 is also associated with significant diminution of both rhizobial and mycorrhizal symbiotic colonization. Additionally, microarray analysis revealed that silencing CDPK1 alters cell wall and defense-related gene expression. We propose that M. truncatula CDPK1 is a key component of one or more signaling pathways that directly or indirectly modulates cell expansion or cell wall synthesis, possibly altering defense gene expression and symbiotic interactions.

MeSH Terms
Arabidopsis Proteins/genetics,metabolism Calcium/metabolism Calcium Signaling/physiology Calcium-Binding Proteins/genetics,isolation & purification,metabolism Cell Wall/enzymology,genetics Gene Expression Regulation, Enzymologic/physiology Gene Expression Regulation, Plant/genetics Gene Silencing/physiology Immunity, Innate/genetics Medicago truncatula/enzymology,genetics,growth & development Plant Diseases/genetics Plant Roots/enzymology,genetics,growth & development Protein Kinases/genetics,isolation & purification,metabolism RNA Interference/physiology Signal Transduction/genetics Symbiosis/genetics
Chemicals
ATCDPK1 protein, Arabidopsis Arabidopsis Proteins Calcium-Binding Proteins Protein Kinases Calcium
Authors & Affiliations
10 authors, click to expand affiliations / ORCID
Ivashuta Sergey
Department of Plant Biology, University of Minesota, St. Paul, Minesota 55108, USA.
Liu Jinyuan
Liu Junqi
Lohar Dasharath P
Haridas Sajeet
Bucciarelli Bruna
VandenBosch Kathryn A
Vance Carroll P
Harrison Maria J
Gantt J Stephen
References (53)
53 references, click to expand
  1. Cytoplasmic free calcium distributions during the development of root hairs of Arabidopsis thaliana.
    Plant J. 1997 Aug;12(2):427-39 PMID: 9301093
  2. Exploring plant genomes by RNA-induced gene silencing.
    Nat Rev Genet. 2003 Jan;4(1):29-38 PMID: 12509751
  3. Analysis of variance for gene expression microarray data.
    J Comput Biol. 2000;7(6):819-37 PMID: 11382364
  4. Ser6 in the maize actin-depolymerizing factor, ZmADF3, is phosphorylated by a calcium-stimulated protein kinase and is essential for the control of functional activity.
    Plant J. 1998 Apr;14(2):187-93 PMID: 9669865
  5. Purification and characterization of peroxidases correlated with lignification in poplar xylem.
    Plant Physiol. 1998 Sep;118(1):125-35 PMID: 9733532
  6. A phosphate transporter from Medicago truncatula involved in the acquisition of phosphate released by arbuscular mycorrhizal fungi.
    Plant Cell. 2002 Oct;14(10):2413-29 PMID: 12368495
  7. Versatile gene-specific sequence tags for Arabidopsis functional genomics: transcript profiling and reverse genetics applications.
    Genome Res. 2004 Oct;14(10B):2176-89 PMID: 15489341
  8. Exploiting the triple response of Arabidopsis to identify ethylene-related mutants.
    Plant Cell. 1990 Jun;2(6):513-23 PMID: 2152173
  9. Computational analysis of microarray data.
    Nat Rev Genet. 2001 Jun;2(6):418-27 PMID: 11389458
  10. Ectopic expression of an Arabidopsis calmodulin-like domain protein kinase-enhanced NADPH oxidase activity and oxidative burst in tomato protoplasts.
    Mol Plant Microbe Interact. 2001 Oct;14(10):1261-4 PMID: 11605966
  11. Depolarization of alfalfa root hair membrane potential by Rhizobium meliloti Nod factors.
    Science. 1992 May 15;256(5059):998-1000 PMID: 10744524
  12. Transcript profiling coupled with spatial expression analyses reveals genes involved in distinct developmental stages of an arbuscular mycorrhizal symbiosis.
    Plant Cell. 2003 Sep;15(9):2106-23 PMID: 12953114
  13. Symbiotic nitrogen fixation and phosphorus acquisition. Plant nutrition in a world of declining renewable resources.
    Plant Physiol. 2001 Oct;127(2):390-7 PMID: 11598215
  14. Oxidative stress-induced calcium signaling in Arabidopsis.
    Plant Physiol. 2004 Jul;135(3):1471-9 PMID: 15247375
  15. Calcium spiking in plant root hairs responding to Rhizobium nodulation signals.
    Cell. 1996 May 31;85(5):673-81 PMID: 8646776
  16. Reduced cellulose synthesis invokes lignification and defense responses in Arabidopsis thaliana.
    Plant J. 2003 May;34(3):351-62 PMID: 12713541
  17. RNA interference in Agrobacterium rhizogenes-transformed roots of Arabidopsis and Medicago truncatula.
    J Exp Bot. 2004 May;55(399):983-92 PMID: 15073217
  18. Agrobacterium rhizogenes-transformed roots of Medicago truncatula for the study of nitrogen-fixing and endomycorrhizal symbiotic associations.
    Mol Plant Microbe Interact. 2001 Jun;14(6):695-700 PMID: 11386364
  19. Calcium signaling through protein kinases. The Arabidopsis calcium-dependent protein kinase gene family.
    Plant Physiol. 2002 Jun;129(2):469-85 PMID: 12068094
  20. Decoding Ca(2+) signals through plant protein kinases.
    Annu Rev Plant Biol. 2004;55:263-88 PMID: 15377221
  21. Phosphorylation of plant actin-depolymerising factor by calmodulin-like domain protein kinase.
    FEBS Lett. 2001 Jun 15;499(1-2):97-100 PMID: 11418120
  22. Nod factor inhibition of reactive oxygen efflux in a host legume.
    Plant Physiol. 2003 Aug;132(4):2196-204 PMID: 12913174
  23. OXI1 kinase is necessary for oxidative burst-mediated signalling in Arabidopsis.
    Nature. 2004 Feb 26;427(6977):858-61 PMID: 14985766
  24. Evolution of signal transduction in intracellular symbiosis.
    Trends Plant Sci. 2002 Nov;7(11):511-8 PMID: 12417152
  25. Medicago truncatula ENOD11: a novel RPRP-encoding early nodulin gene expressed during mycorrhization in arbuscule-containing cells.
    Mol Plant Microbe Interact. 2001 Jun;14(6):737-48 PMID: 11386369
  26. Knocking on the heaven's wall: pathogenesis of and resistance to biotrophic fungi at the cell wall.
    Curr Opin Plant Biol. 2004 Aug;7(4):377-83 PMID: 15231259
  27. Arabidopsis thaliana root non-selective cation channels mediate calcium uptake and are involved in growth.
    Plant J. 2002 Dec;32(5):799-808 PMID: 12472694
  28. Reactive oxygen species activation of plant Ca2+ channels. A signaling mechanism in polar growth, hormone transduction, stress signaling, and hypothetically mechanotransduction.
    Plant Physiol. 2004 Jun;135(2):702-8 PMID: 15208417
  29. Four genes of Medicago truncatula controlling components of a nod factor transduction pathway.
    Plant Cell. 2000 Sep;12(9):1647-66 PMID: 11006338
  30. MOLECULAR AND CELLULAR ASPECTS OF THE ARBUSCULAR MYCORRHIZAL SYMBIOSIS.
    Annu Rev Plant Physiol Plant Mol Biol. 1999 Jun;50:361-389 PMID: 15012214
  31. Glycosyltransferases and cell wall biosynthesis: novel players and insights.
    Curr Opin Plant Biol. 2004 Jun;7(3):285-95 PMID: 15134749
  32. Resistance gene-dependent activation of a calcium-dependent protein kinase in the plant defense response.
    Plant Cell. 2000 May;12(5):803-16 PMID: 10810151
  33. Unstable F-actin specifies the area and microtubule direction of cell expansion in Arabidopsis root hairs.
    Plant Cell. 2003 Jan;15(1):285-92 PMID: 12509537
  34. CDPK-mediated signalling pathways: specificity and cross-talk.
    J Exp Bot. 2004 Jan;55(395):181-8 PMID: 14623901
  35. A Ca2+/calmodulin-dependent protein kinase required for symbiotic nodule development: Gene identification by transcript-based cloning.
    Proc Natl Acad Sci U S A. 2004 Mar 30;101(13):4701-5 PMID: 15070781
  36. LysM domain receptor kinases regulating rhizobial Nod factor-induced infection.
    Science. 2003 Oct 24;302(5645):630-3 PMID: 12947035
  37. Reactive oxygen species produced by NADPH oxidase regulate plant cell growth.
    Nature. 2003 Mar 27;422(6930):442-6 PMID: 12660786
  38. SIGNALING TO THE ACTIN CYTOSKELETON IN PLANTS.
    Annu Rev Plant Physiol Plant Mol Biol. 2000 Jun;51:257-288 PMID: 15012193
  39. Infection and invasion of roots by symbiotic, nitrogen-fixing rhizobia during nodulation of temperate legumes.
    Microbiol Mol Biol Rev. 2004 Jun;68(2):280-300 PMID: 15187185
  40. A putative Ca2+ and calmodulin-dependent protein kinase required for bacterial and fungal symbioses.
    Science. 2004 Feb 27;303(5662):1361-4 PMID: 14963335
  41. Calcium, kinases and nodulation signalling in legumes.
    Nat Rev Mol Cell Biol. 2004 Jul;5(7):566-76 PMID: 15232574
  42. Regulation of the plant defence response in arbuscular mycorrhizal symbiosis.
    J Exp Bot. 2002 Jun;53(373):1377-86 PMID: 12021285
  43. The TIGR gene indices: reconstruction and representation of expressed gene sequences.
    Nucleic Acids Res. 2000 Jan 1;28(1):141-5 PMID: 10592205
  44. Refined analysis of early symbiotic steps of the Rhizobium-Medicago interaction in relationship with microtubular cytoskeleton rearrangements.
    Development. 1999 Aug;126(16):3617-28 PMID: 10409507
  45. Significance analysis of microarrays applied to the ionizing radiation response.
    Proc Natl Acad Sci U S A. 2001 Apr 24;98(9):5116-21 PMID: 11309499
  46. Environmentally induced plasticity of root hair development in Arabidopsis.
    Plant Physiol. 2004 Jan;134(1):409-19 PMID: 14730071
  47. Suppression of plant defence in rhizobia-legume symbiosis.
    Trends Plant Sci. 2002 Oct;7(10):440-4 PMID: 12399178
  48. Actin polymerization processes in plant cells.
    Curr Opin Plant Biol. 2002 Dec;5(6):502-6 PMID: 12393012
  49. Involvement of the mitogen-activated protein kinase SIMK in regulation of root hair tip growth.
    EMBO J. 2002 Jul 1;21(13):3296-306 PMID: 12093731
  50. Molecular mechanisms controlling actin filament dynamics in nonmuscle cells.
    Annu Rev Biophys Biomol Struct. 2000;29:545-76 PMID: 10940259
  51. Calcium in plants.
    Ann Bot. 2003 Oct;92(4):487-511 PMID: 12933363
  52. Stepwise understanding of root development.
    Curr Opin Plant Biol. 2005 Feb;8(1):71-6 PMID: 15653403
  53. Pectin methylesterases: cell wall enzymes with important roles in plant physiology.
    Trends Plant Sci. 2001 Sep;6(9):414-9 PMID: 11544130
Article Info
Journal
The Plant cell
Abbr.
Plant Cell
ISSN
1040-4651
DOI
10.1105/tpc.105.035394
Published
2005-11-00
Epub
2005-00-30
Pages
2911-21
Language
English
Region
England
NLM ID
9208688
PMCID
PMC1276019
Subset
IM
External Links
PubMed source DOI: 10.1105/tpc.105.035394 PMC Full Text View journal details
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