Abstract
Vascular endothelial growth factor-receptors (VEGF-Rs) are pivotal regulators of vascular development, but a specific role for these receptors in the formation of heart valves has not been identified. We took advantage of small molecule inhibitors of VEGF-R signaling and showed that blocking VEGF-R signaling with receptor selective tyrosine kinase inhibitors, PTK 787 and AAC 787, from 17-21 hr post-fertilization (hpf) in zebrafish embryos resulted in a functional and structural defect in cardiac valve development. Regurgitation of blood between the two chambers of the heart, as well as a loss of cell-restricted expression of the valve differentiation markers notch 1b and bone morphogenetic protein-4 (bmp-4), was readily apparent in treated embryos. In addition, microangiography revealed a loss of a definitive atrioventricular constriction in treated embryos. Taken together, these data demonstrate a novel function for VEGF-Rs in the endocardial endothelium of the developing cardiac valve.
MeSH Terms
Animals
Bone Morphogenetic Protein 4
Bone Morphogenetic Proteins/biosynthesis,genetics
Cell Nucleus/metabolism
Cells, Cultured
Gene Expression Regulation, Developmental/drug effects
Heart Valves/abnormalities,drug effects,embryology,physiology
Humans
NFATC Transcription Factors/metabolism
Phthalazines/pharmacology
Pyridines/pharmacology
Receptor, Notch1/biosynthesis,genetics
Receptors, Vascular Endothelial Growth Factor/physiology
Signal Transduction/drug effects,physiology
Zebrafish/embryology,physiology
Zebrafish Proteins
Chemicals
BMP4 protein, human
Bone Morphogenetic Protein 4
Bone Morphogenetic Proteins
NFATC Transcription Factors
Phthalazines
Pyridines
Receptor, Notch1
Zebrafish Proteins
bmp4 protein, zebrafish
vatalanib
Receptors, Vascular Endothelial Growth Factor
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Lee You Mie
Vascular Biology Program, Department of Surgery, Children's Hospital, Boston, Massachusetts 02115, USA.
Cope John J
Ackermann Gabriele E
Goishi Katsutoshi
Armstrong Ehrin J
Paw Barry H
Bischoff Joyce
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