Abstract
The transcription factor Spo0A is a master regulator for entry into sporulation in the bacterium Bacillus subtilis, but it has been uncertain whether activation of Spo0A is sufficient to trigger development. Spo0A, a member of the response regulator family of gene-control proteins, is activated by phosphorylation via a multicomponent phosphorelay in response to conditions of nutrient limitation. We now report that sporulation can be triggered with high efficiency in cells in the exponential phase of growth in rich medium by artificial induction of the synthesis of any one of three histidine kinases that feed phosphoryl groups into the relay. We further show that the levels of Spo0A protein and activity increase gradually over the first 2 h of sporulation both under conditions of nutrient limitation and in response to induction of kinase synthesis. Evidence indicates that this gradual increase in Spo0A protein and activity plays a critical role in triggering sporulation and requires the action of the phosphorelay.
MeSH Terms
Artificial Gene Fusion
Bacillus subtilis/genetics,growth & development,physiology
Bacterial Proteins/biosynthesis
Dose-Response Relationship, Drug
Enzyme Induction
Fluorescent Dyes
Gene Expression Regulation, Bacterial
Isopropyl Thiogalactoside/pharmacology
Kinetics
Microscopy, Fluorescence
Phosphotransferases/biosynthesis
Promoter Regions, Genetic
Protein Kinases/biosynthesis
Pyridinium Compounds
Quaternary Ammonium Compounds
Spores, Bacterial/physiology
Time Factors
Transcription Factors/metabolism
Transcription, Genetic
beta-Galactosidase/metabolism
Chemicals
Bacterial Proteins
FM 4-64
Fluorescent Dyes
Pyridinium Compounds
Quaternary Ammonium Compounds
Transcription Factors
kinA protein, Bacillus subtilis
Isopropyl Thiogalactoside
Phosphotransferases
Protein Kinases
kinB protein, Bacillus subtilis
beta-Galactosidase
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Fujita Masaya
Department of Molecular and Cellular Biology, Harvard University, Cambridge, Massachusetts 02138, USA.
Losick Richard
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