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PMID: 16143609 Published · ppublish English Comparative Study Journal Article Research Support, U.S. Gov't, Non-P.H.S.

Allele-specific, bidirectional silencing of an alcohol dehydrogenase gene in different organs of interspecific diploid cotton hybrids.

Genetics ·Vol. 171 ·No. 4 ·2005-12-00 ·Pages 2139-42

Adams KL, Wendel JF

Abstract

Interspecific hybridization is a common and important process that generates phenotypic novelty and can lead to hybrid speciation as well as to changes in gene expression. Using two different interspecific cotton (Gossypium) diploid hybrids, we show organ-specific, bidirectional allelic silencing at a heterozygous Adh locus, with alternate alleles being silenced in leaves and many floral organs, respectively. These results show that developmental regulation of gene expression is changed immediately upon hybridization between diploid species, possibly due to epigenetic factors or regulatory mismatch.

MeSH Terms
Alcohol Dehydrogenase/genetics,metabolism DNA Primers Flowers/genetics Gene Expression Gene Expression Regulation, Developmental Gene Silencing Gossypium/genetics Heterozygote Hybridization, Genetic Plant Leaves/genetics Reverse Transcriptase Polymerase Chain Reaction
Chemicals
DNA Primers Alcohol Dehydrogenase
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Adams Keith L
UBC Botanical Garden, Centre for Plant Research and Department of Botany, University of British Columbia, Vancouver, BC V6T 1Z4, Canada. keitha@interchange.ubc.ca
Wendel Jonathan F
References (22)
22 references, click to expand
  1. Natural hybridization and the evolution of domesticated, pest and disease organisms.
    Mol Ecol. 2004 May;13(5):997-1007 PMID: 15078439
  2. Detection of regulatory variation in mouse genes.
    Nat Genet. 2002 Nov;32(3):432-7 PMID: 12410233
  3. Dosage balance in gene regulation: biological implications.
    Trends Genet. 2005 Apr;21(4):219-26 PMID: 15797617
  4. Hybrid speciation in plants: new insights from molecular studies.
    New Phytol. 2005 Feb;165(2):411-23 PMID: 15720652
  5. Simultaneous genotyping, gene-expression measurement, and detection of allele-specific expression with oligonucleotide arrays.
    Genome Res. 2005 Feb;15(2):284-91 PMID: 15687292
  6. Sequence elimination and cytosine methylation are rapid and reproducible responses of the genome to wide hybridization and allopolyploidy in wheat.
    Plant Cell. 2001 Aug;13(8):1749-59 PMID: 11487690
  7. Allelic variation of gene expression in maize hybrids.
    Plant Cell. 2004 Jul;16(7):1707-16 PMID: 15194819
  8. Genome-wide mRNA profiling reveals heterochronic allelic variation and a new imprinted gene in hybrid maize endosperm.
    Plant J. 2003 Oct;36(1):30-44 PMID: 12974809
  9. Organ-specific silencing of duplicated genes in a newly synthesized cotton allotetraploid.
    Genetics. 2004 Dec;168(4):2217-26 PMID: 15371349
  10. Quantitative analysis of transcript accumulation from genes duplicated by polyploidy using cDNA-SSCP.
    Biotechniques. 2003 Apr;34(4):726-30, 732, 734 PMID: 12703297
  11. Mobilization of the active MITE transposons mPing and Pong in rice by introgression from wild rice (Zizania latifolia Griseb.).
    Mol Biol Evol. 2005 Apr;22(4):976-90 PMID: 15647520
  12. Genes duplicated by polyploidy show unequal contributions to the transcriptome and organ-specific reciprocal silencing.
    Proc Natl Acad Sci U S A. 2003 Apr 15;100(8):4649-54 PMID: 12665616
  13. Nonadditive gene expression in diploid and triploid hybrids of maize.
    Genetics. 2005 Jan;169(1):389-97 PMID: 15489529
  14. Cloning and characterization of leaf cDNAs that are differentially expressed between wheat hybrids and their parents.
    Mol Genet Genomics. 2003 Nov;270(3):281-6 PMID: 14551762
  15. Major ecological transitions in wild sunflowers facilitated by hybridization.
    Science. 2003 Aug 29;301(5637):1211-6 PMID: 12907807
  16. Paralogs in polyploids: one for all and all for one?
    Plant Cell. 2005 Jan;17(1):4-11 PMID: 15632052
  17. Genetic and epigenetic consequences of recent hybridization and polyploidy in Spartina (Poaceae).
    Mol Ecol. 2005 Apr;14(4):1163-75 PMID: 15773943
  18. Role of Gene Interactions in Hybrid Speciation: Evidence from Ancient and Experimental Hybrids
    Science. 1996 May 3;272(5262):741-5 PMID: 8662570
  19. Development of anonymous cDNA microarrays to study changes to the Senecio floral transcriptome during hybrid speciation.
    Mol Ecol. 2005 Jul;14(8):2493-510 PMID: 15969730
  20. Transgressive segregation, adaptation and speciation.
    Heredity (Edinb). 1999 Oct;83 ( Pt 4):363-72 PMID: 10583537
  21. Retrotransposon activation followed by rapid repression in introgressed rice plants.
    Genome. 2000 Oct;43(5):874-80 PMID: 11081978
  22. Evolutionary changes in cis and trans gene regulation.
    Nature. 2004 Jul 1;430(6995):85-8 PMID: 15229602
Article Info
Journal
Genetics
Abbr.
Genetics
ISSN
0016-6731
DOI
10.1534/genetics.105.047357
Email
keitha@interchange.ubc.ca
Published
2005-12-00
Epub
2005-00-02
Pages
2139-42
Language
English
Region
United States
NLM ID
0374636
PMCID
PMC1456131
Subset
IM
External Links
PubMed source DOI: 10.1534/genetics.105.047357 PMC Full Text View journal details
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