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PMID: 16113265 Published · ppublish English Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

The Aspergillus fumigatus siderophore biosynthetic gene sidA, encoding L-ornithine N5-oxygenase, is required for virulence.

Infection and immunity ·Vol. 73 ·No. 9 ·2005-09-00 ·Pages 5493-503

Hissen AH, Wan AN, Warwas ML, Pinto LJ, Moore MM

Abstract

Aspergillus fumigatus is the leading cause of invasive mold infection and is a serious problem in immunocompromised populations worldwide. We have previously shown that survival of A. fumigatus in serum may be related to secretion of siderophores. In this study, we identified and characterized the sidA gene of A. fumigatus, which encodes l-ornithine N(5)-oxygenase, the first committed step in hydroxamate siderophore biosynthesis. A. fumigatus sidA codes for a protein of 501 amino acids with significant homology to other fungal l-ornithine N(5)-oxygenases. A stable DeltasidA strain was created by deletion of A. fumigatus sidA. This strain was unable to synthesize the siderophores N',N",N'''-triacetylfusarinine C (TAF) and ferricrocin. Growth of the DeltasidA strain was the same as that of the wild type in rich media; however, the DeltasidA strain was unable to grow in low-iron defined media or media containing 10% human serum unless supplemented with TAF or ferricrocin. No significant differences in ferric reduction activities were observed between the parental strain and the DeltasidA strain, indicating that blocking siderophore secretion did not result in upregulation of this pathway. Unlike the parental strain, the DeltasidA strain was unable to remove iron from human transferrin. A rescued strain (DeltasidA + sidA) was constructed; it produced siderophores and had the same growth as the wild type on iron-limited media. Unlike the wild-type and rescued strains, the DeltasidA strain was avirulent in a mouse model of invasive aspergillosis, indicating that sidA is necessary for A. fumigatus virulence.

MeSH Terms
Amino Acid Sequence Animals Aspergillosis/enzymology,microbiology,pathology Aspergillus fumigatus/genetics,pathogenicity Disease Models, Animal Ferrichrome/analogs & derivatives,metabolism Humans Hydroxamic Acids/metabolism Lung/microbiology,pathology Lung Diseases, Fungal/enzymology,microbiology,pathology Mice Mixed Function Oxygenases/genetics,physiology Molecular Sequence Data Oxidation-Reduction Sequence Alignment Siderophores/biosynthesis Virulence
Chemicals
Hydroxamic Acids Siderophores fusarinine Ferrichrome ferricrocin Mixed Function Oxygenases ornithine N5-oxygenase
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Hissen Anna H T
Department of Biological Sciences, Simon Fraser University, 8888 University Drive, Burnaby, British Columbia V5A 1S6, Canada. mmoore@sfu.ca.
Wan Adrian N C
Warwas Mark L
Pinto Linda J
Moore Margo M
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Article Info
Journal
Infection and immunity
Abbr.
Infect Immun
ISSN
0019-9567
Published
2005-09-00
Pages
5493-503
Language
English
Region
United States
NLM ID
0246127
PMCID
PMC1231119
Subset
IM
Grants
Wellcome Trust · United Kingdom
Databases
GENBANK
AY819708
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