Abstract
Neuronal development requires highly coordinated regulation of the cytoskeleton within the developing axon. This dynamic regulation manifests itself in axonal branching, turning and pathfinding, presynaptic differentiation, and growth cone collapse and extension. Semaphorin 3A (Sema3A), a secreted guidance cue that primarily functions to repel axons from inappropriate targets, induces cytoskeletal rearrangements that result in growth cone collapse. These effects require intra-axonal messenger RNA translation. Here we show that transcripts for RhoA, a small guanosine triphosphatase (GTPase) that regulates the actin cytoskeleton, are localized to developing axons and growth cones, and this localization is mediated by an axonal targeting element located in the RhoA 3' untranslated region (UTR). Sema3A induces intra-axonal translation of RhoA mRNA, and this local translation of RhoA is necessary and sufficient for Sema3A-mediated growth cone collapse. These studies indicate that local RhoA translation regulates the neuronal cytoskeleton and identify a new mechanism for the regulation of RhoA signalling.
MeSH Terms
3' Untranslated Regions/genetics,metabolism
Animals
Axons/drug effects,metabolism
Cell Line
Cells, Cultured
Cricetinae
Genes, Reporter/genetics
Growth Cones/drug effects,metabolism
Protein Biosynthesis
RNA Transport
RNA, Small Interfering/genetics,metabolism
Rats
Semaphorin-3A/metabolism,pharmacology
rhoA GTP-Binding Protein/biosynthesis,genetics,metabolism
Chemicals
3' Untranslated Regions
RNA, Small Interfering
Semaphorin-3A
rhoA GTP-Binding Protein
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Wu Karen Y
Department of Pharmacology, Weill Medical College, Cornell University, New York, NY 10021, USA.
Hengst Ulrich
Department of Pharmacology, Weill Medical College, Cornell University, New York, NY 10021, USA.
Cox Llewellyn J
Department of Pharmacology, Weill Medical College, Cornell University, New York, NY 10021, USA.
Macosko Evan Z
Department of Pharmacology, Weill Medical College, Cornell University, New York, NY 10021, USA.
Jeromin Andreas
Department of Neuroscience, Baylor College of Medicine, Houston, TX 77030, USA.
Urquhart Erica R
Department of Pharmacology, Weill Medical College, Cornell University, New York, NY 10021, USA.
Jaffrey Samie R
Department of Pharmacology, Weill Medical College, Cornell University, New York, NY 10021, USA.
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