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PMID: 16094968 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

The effect of three rad genes on survival, inter- and intragenic mitotic recombination in Saccharomyces. I. UV irradiation without photoreactivation or liquid-holding post-treatment.

Molecular & general genetics : MGG ·Vol. 136 ·No. 1 ·1975-00-00 ·Pages 75-86

Kowalski S, Laskowski W

Abstract

The effect of UV irradiation on the survival, inter- and intragenic mitotic recombination of 3 diploid UV sensitive Saccharomyces mutants was studied and compared with the wild type RAD. These strains, homozygous for either the RAD, r1s rad 9-4, or rad 2-20 gene, have DRF values for survival of 1:1.6:3:20.6 respectively, at LD1. Their recombination behaviour is not correlated to their survival characteristics. The RAD, r1s, and rad 2-20 strains showed UV induced mitotic inter- and intragenic recombinants; the induction in the r1s diploid is ca. 100 times greater for both the inter- and intragenic recombinants than in the RAD strain. The rad 9-4 diploid produced no UV induced mitotic recombinants whatsoever, and is therefore considered to be a rec- mutation.

MeSH Terms
Genes, Fungal Mutation Recombination, Genetic/genetics,radiation effects Saccharomyces/genetics,radiation effects Ultraviolet Rays
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Kowalski S
Zentralinstitut für Biochemie und Biophysik der Freien Universität Berlin.
Laskowski W
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Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00275450
Published
1975-00-00
Pages
75-86
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00275450
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