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PMID: 16085804 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Impact of plant species and site on rhizosphere-associated fungi antagonistic to Verticillium dahliae kleb.

Applied and environmental microbiology ·Vol. 71 ·No. 8 ·2005-08-00 ·Pages 4203-13

Berg G, Zachow C, Lottmann J, Götz M, Costa R, Smalla K

Abstract

Fungi with antagonistic activity toward plant pathogens play an essential role in plant growth and health. To analyze the effects of the plant species and the site on the abundance and composition of fungi with antagonistic activity toward Verticillium dahliae, fungi were isolated from oilseed rape and strawberry rhizosphere and bulk soil from three different locations in Germany over two growing seasons. A total of 4,320 microfungi screened for in vitro antagonism toward Verticillium resulted in 911 active isolates. This high proportion of fungi antagonistic toward the pathogen V. dahliae was found for bulk and rhizosphere soil at all sites. A plant- and site-dependent specificity of the composition of antagonistic morphotypes and their genotypic diversity was found. The strawberry rhizosphere was characterized by preferential occurrence of Penicillium and Paecilomyces isolates and low numbers of morphotypes (n = 31) and species (n = 13), while Monographella isolates were most frequently obtained from the rhizosphere of oilseed rape, for which higher numbers of morphotypes (n = 41) and species (n = 17) were found. Trichoderma strains displayed high diversity in all soils, but a high degree of plant specificity was shown by BOX-PCR fingerprints. The diversity of rhizosphere-associated antagonists was lower than that of antagonists in bulk soil, suggesting that some fungi were specifically enriched in each rhizosphere. A broad spectrum of new Verticillium antagonists was identified, and the implications of the data for biocontrol applications are discussed.

MeSH Terms
Antibiosis Brassica napus/microbiology Crops, Agricultural/classification,microbiology DNA, Fungal/analysis DNA, Ribosomal/analysis Ecosystem Fragaria/microbiology Fungi/classification,genetics,growth & development Genotype Molecular Sequence Data Plant Diseases/microbiology Plant Roots/microbiology RNA, Ribosomal, 18S/genetics Soil Microbiology Species Specificity Verticillium/growth & development,pathogenicity
Chemicals
DNA, Fungal DNA, Ribosomal RNA, Ribosomal, 18S
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Berg Gabriele
University of Rostock, Fachbereich Biowissenschaften, Microbiology, D-18051 Rostock, Germany. gabriele.berg@uni-rostock.de
Zachow Christin
Lottmann Jana
Götz Monika
Costa Rodrigo
Smalla Kornelia
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Article Info
Journal
Applied and environmental microbiology
Abbr.
Appl Environ Microbiol
ISSN
0099-2240
DOI
10.1128/AEM.71.8.4203-4213.2005
Email
gabriele.berg@uni-rostock.de
Published
2005-08-00
Pages
4203-13
Language
English
Region
United States
NLM ID
7605801
PMCID
PMC1183293
Subset
IM
Databases
GENBANK
AJ745088, AJ745089, AJ746347, AJ746348, AJ746349, AJ746350, AJ746351, AJ746352, AJ746353, AJ746354, AJ746355, AJ746356, AJ748271, AJ748272, AJ748273, AJ748274, AJ748275, AJ748759, AJ748760, AJ748761, AJ748762, AJ748763, AJ781112, AJ781113, AJ781114, AJ781115, AJ781116, AJ781423, AJ781424, AJ781425, AJ783407, AJ783408, AJ783409, AJ783410, AJ783411, AJ783412, AJ783413, AJ783414, AJ783415, AJ783416, AJ783417, AJ783920, AJ783921, AJ783922, AJ783923, AJ783924, AJ783925, AJ783926, AJ783927, AJ783928, AJ783929, AJ783930, AJ783931, AJ783932, AJ783933, AJ783934, AJ783935, AJ783936, AJ783937, AJ783938, AJ783939, AJ783940, AJ783941, AJ783942, AJ783943, AJ783944, AJ783945, AJ783946, AJ783947, AJ783948, AJ783949, AJ784284, AJ784285, AJ784286, AJ784287
External Links
PubMed source DOI: 10.1128/AEM.71.8.4203-4213.2005 PMC Full Text View journal details
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