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PMID: 16076400 Published · epublish English Journal Article Research Support, Non-U.S. Gov't

The influence of acetyl phosphate on DspA signalling in the Cyanobacterium Synechocystis sp. PCC6803.

BMC microbiology ·Vol. 5 ·2005-08-02 ·Pages 47

Morrison SS, Mullineaux CW, Ashby MK

Abstract

The dspA (hik33) gene, coding for a putative sensory histidine kinase, is conserved in plastids (ycf26) and cyanobacteria. It has been linked with a number of different stress responses in cyanobacteria. We constructed an insertional mutant of dspA (ycf26) in Synechocystis 6803. We found little phenotypic effect during nitrogen starvation. However, when the mutation was combined with deletion of the pta gene coding for phosphotransacetylase, a more significant phenotype was observed. Under nitrogen starvation, the pta/dspA double mutant degrades its phycobilisomes less than the wild type and still has about half of its chlorophyll-protein complexes. Our data indicates that acetyl-phosphate-dependent phosphorylation of response regulator(s) overlaps with DspA-dependent signalling of the degradation of chlorophyll-protein complexes (and to a lesser extent phycobilisomes) in Synechocystis 6803.

MeSH Terms
Bacterial Proteins/metabolism Base Sequence Culture Media DNA, Bacterial/chemistry,genetics Histidine Kinase Open Reading Frames Organophosphates/pharmacology Polymerase Chain Reaction Protein Kinases/metabolism Signal Transduction/drug effects,physiology Synechocystis/drug effects,genetics,growth & development,physiology
Chemicals
Bacterial Proteins Culture Media DNA, Bacterial Organophosphates acetyl phosphate Protein Kinases Histidine Kinase
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Morrison S Shawn
Department of Basic Medical Sciences, Biochemistry Section, the University of the West Indies, Mona Campus, Kingston 7, Jamaica. sshawn58@hotmail.com
Mullineaux Conrad W
Ashby Mark K
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Article Info
Journal
BMC microbiology
Abbr.
BMC Microbiol
ISSN
1471-2180
DOI
10.1186/1471-2180-5-47
Email
sshawn58@hotmail.com
Published
2005-08-02
Epub
2005-00-02
Pages
47
Language
English
Region
England
NLM ID
100966981
PMCID
PMC1192802
Subset
IM
External Links
PubMed source DOI: 10.1186/1471-2180-5-47 PMC Full Text View journal details
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