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PMID: 16061821 Published · ppublish English Comparative Study Journal Article Research Support, N.I.H., Intramural

Mass of the postsynaptic density and enumeration of three key molecules.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 102 ·No. 32 ·2005-08-09 ·Pages 11551-6

Chen X, Vinade L, Leapman RD, Petersen JD, Nakagawa T, Phillips TM, Sheng M, Reese TS

Abstract

The total molecular mass of individual postsynaptic densities (PSDs) isolated from rat forebrain was measured by scanning transmission EM. PSDs had a mean diameter of 360 nm and molecular mass of 1.10 +/- 0.36 GDa. Because the mass represents the sum of the molecular masses of all of the molecules comprising a PSD, it becomes possible to derive the number of copies of each protein, once its relative mass contribution is known. Mass contributions of PSD-95, synapse-associated protein (SAP)97, and alpha-Ca2+/calmodulin-dependent protein kinase II (CaMKII) were determined by quantitative gel electrophoresis of PSD fractions. The number of PSD-95 molecules per average PSD, contributing 2.3% of the mass of the PSD, was calculated to be 300, whereas the number of SAP97 molecules, contributing 0.9% of the mass of the PSD, was 90. The alpha-CaMKII holoenzymes, which contribute 6% of the mass when brains are homogenized within 2 min of interrupting blood flow, have 80 holoenzymes associated with a typical PSD. When blood flow is interrupted 15 min before homogenization, the average mass of PSDs increases by approximately 40%. The additional alpha-CaMKII associated with PSDs accounts for up to 20% of this mass increase, representing the addition of 100-200 alpha-CaMKII holoenzymes.

MeSH Terms
Adaptor Proteins, Signal Transducing/chemistry,isolation & purification Animals Calcium-Calmodulin-Dependent Protein Kinases/chemistry,isolation & purification,ultrastructure Electrophoresis Membrane Proteins/chemistry,isolation & purification,ultrastructure Microscopy, Electron Multiprotein Complexes/chemistry,isolation & purification,ultrastructure Nerve Tissue Proteins/chemistry,isolation & purification,ultrastructure Prosencephalon/cytology,metabolism Rats
Chemicals
Adaptor Proteins, Signal Transducing Dlg1 protein, rat Membrane Proteins Multiprotein Complexes Nerve Tissue Proteins postsynaptic density proteins Calcium-Calmodulin-Dependent Protein Kinases
Authors & Affiliations
8 authors, click to expand affiliations / ORCID
Chen Xiaobing
Laboratory of Neurobiology, National Institute of Neurological Disorders and Stroke, National Institutes of Health, 36 Convent Drive, MSC 4062, Bethesda, MD 20892, USA.
Vinade Lucia
Leapman Richard D
Petersen Jennifer D
Nakagawa Terunaga
Phillips Terry M
Sheng Morgan
Reese Thomas S
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.0505359102
Published
2005-08-09
Epub
2005-00-01
Pages
11551-6
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC1182136
Subset
IM
Grants
Intramural NIH HHS · United States
External Links
PubMed source DOI: 10.1073/pnas.0505359102 PMC Full Text View journal details
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