Abstract
The protective antigen component of anthrax toxin forms a homoheptameric pore in the endosomal membrane, creating a narrow passageway for the enzymatic components of the toxin to enter the cytosol. We found that, during conversion of the heptameric precursor to the pore, the seven phenylalanine-427 residues converged within the lumen, generating a radially symmetric heptad of solvent-exposed aromatic rings. This "phi-clamp" structure was required for protein translocation and comprised the major conductance-blocking site for hydrophobic drugs and model cations. We conclude that the phi clamp serves a chaperone-like function, interacting with hydrophobic sequences presented by the protein substrate as it unfolds during translocation.
MeSH Terms
Amino Acid Sequence
Amino Acid Substitution
Antigens, Bacterial/chemistry,genetics,metabolism
Bacillus anthracis/chemistry,metabolism
Bacterial Toxins/chemistry,genetics,metabolism
Binding Sites
Cell Membrane/metabolism
Cytosol/metabolism
Electron Spin Resonance Spectroscopy
Endosomes/metabolism
Hydrogen-Ion Concentration
Hydrophobic and Hydrophilic Interactions
Lipid Bilayers/metabolism
Models, Biological
Models, Molecular
Molecular Sequence Data
Mutagenesis
Onium Compounds/metabolism
Organophosphorus Compounds/metabolism
Phenylalanine/chemistry
Protein Conformation
Protein Folding
Quaternary Ammonium Compounds/metabolism
Chemicals
Antigens, Bacterial
Bacterial Toxins
Lipid Bilayers
Onium Compounds
Organophosphorus Compounds
Quaternary Ammonium Compounds
anthrax toxin
Phenylalanine
tetrabutylammonium
tetraphenylphosphonium
Authors & Affiliations
8 authors, click to expand affiliations / ORCID
Krantz Bryan A
Department of Microbiology and Molecular Genetics, Harvard Medical School, 200 Longwood Avenue, Boston, MA 02115, USA.
Melnyk Roman A
Zhang Sen
Juris Stephen J
Lacy D Borden
Wu Zhengyan
Finkelstein Alan
Collier R John
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