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PMID: 16023891 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Usefulness of repeated GenomiPhi, a phi29 DNA polymerase-based rolling circle amplification kit, for generation of large amounts of plasmid DNA.

Biomolecular engineering ·Vol. 22 ·No. 4 ·2005-10-00 ·Pages 129-32

Sato M, Ohtsuka M, Ohmi Y

Abstract

The GenomiPhi DNA Amplification Kit employs rolling circle amplification (RCA) using phi29 polymerase, dNTPs, and random hexamers. We demonstrated that repeated RCA (at least three times) is useful for high-fidelity amplification of large amounts of plasmid DNA.

MeSH Terms

Bacillus Phages/genetics Blotting, Southern DNA/genetics DNA, Circular/genetics DNA-Directed DNA Polymerase/chemistry,genetics Ethidium/pharmacology Genetic Techniques Green Fluorescent Proteins/metabolism Nucleic Acid Amplification Techniques/methods Plasmids/genetics,metabolism Software Templates, Genetic Time Factors Ultraviolet Rays

Chemicals

DNA, Circular Green Fluorescent Proteins DNA DNA-Directed DNA Polymerase Ethidium

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Sato Masahiro

The Institute of Medical Sciences, Tokai University, Bohseidai, Isehara, Kanagawa 259-1193, Japan. masasato@is.icc.u-tokai.ac.jp

Ohtsuka Masato

Ohmi Yuhsuke

Article Info

Journal

Biomolecular engineering

Abbr.

Biomol Eng

ISSN

1389-0344

DOI

10.1016/j.bioeng.2005.05.001

masasato@is.icc.u-tokai.ac.jp

Published

2005-10-00

Pages

129-32

Language

English

Region

Netherlands

NLM ID

100928062

Subset

External Links

PubMed source DOI: 10.1016/j.bioeng.2005.05.001

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Usefulness of repeated GenomiPhi, a phi29 DNA polymerase-based rolling circle amplification kit, for generation of large amounts of plasmid DNA.

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