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PMID: 16020774 Published · ppublish English Journal Article Research Support, N.I.H., Extramural

Highly efficient sex chromosome interchanges produced by I-CreI expression in Drosophila.

Genetics ·Vol. 171 ·No. 3 ·2005-11-00 ·Pages 1103-14

Maggert KA, Golic KG

Abstract

The homing endonuclease I-CreI recognizes a site in the gene encoding the 23S rRNA of Chlamydomonas reinhardtii. A very similar sequence is present in the 28S rRNA genes that are located on the X and Y chromosomes of Drosophila melanogaster. In this work we show that I-CreI expression in Drosophila is capable of causing induced DNA damage and eliciting cell cycle arrest. Expression also caused recombination between the X and Y chromosomes in the heterochromatic regions where the rDNA is located, presumably as a result of a high frequency of double-strand breaks in these regions. Approximately 20% of the offspring of males expressing I-CreI showed exceptional inheritance of X- and Y-linked markers, consistent with chromosome exchange at rDNA loci. Cytogenetic analysis confirmed the structures of many of these products. Exchange between the X and Y chromosomes can be induced in males and females to produce derivative-altered Y chromosomes, attached-XY, and attached-X chromosomes. This method has advantages over the traditional use of X rays for generating X-Y interchanges because it is very frequent and it generates predictable products.

MeSH Terms
Animals DNA Damage/physiology DNA Restriction Enzymes/biosynthesis,genetics Drosophila melanogaster/cytology,enzymology,genetics Female Male Mitosis/physiology Recombination, Genetic/physiology X Chromosome/physiology Y Chromosome/physiology
Chemicals
DNA Restriction Enzymes endodeoxyribonuclease CreI
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Maggert Keith A
Department of Biology, University of Utah, Salt Lake City, Utah 84112.
Golic Kent G
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Article Info
Journal
Genetics
Abbr.
Genetics
ISSN
0016-6731
DOI
10.1534/genetics.104.040071
Published
2005-11-00
Epub
2005-00-14
Pages
1103-14
Language
English
Region
United States
NLM ID
0374636
PMCID
PMC1456814
Subset
IM
Grants
NIGMS NIH HHS · F32 GM065777 · United States
NIGMS NIH HHS · R01 GM065604 · United States
NIGMS NIH HHS · GM-065604 · United States
NIGMS NIH HHS · GM-65777 · United States
External Links
PubMed source DOI: 10.1534/genetics.104.040071 PMC Full Text View journal details
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