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PMID: 16009997 Published · ppublish English Journal Article

Serial analysis of gene expression study of a hybrid rice strain (LYP9) and its parental cultivars.

Plant physiology ·Vol. 138 ·No. 3 ·2005-07-00 ·Pages 1216-31

Bao J, Lee S, Chen C, Zhang X, Zhang Y, Liu S, Clark T, Wang J, Cao M, Yang H, Wang SM, Yu J

Abstract

Using the serial analysis of gene expression technique, we surveyed transcriptomes of three major tissues (panicles, leaves, and roots) of a super-hybrid rice (Oryza sativa) strain, LYP9, in comparison to its parental cultivars, 93-11 (indica) and PA64s (japonica). We acquired 465,679 tags from the serial analysis of gene expression libraries, which were consolidated into 68,483 unique tags. Focusing our initial functional analyses on a subset of the data that are supported by full-length cDNAs and the tags (genes) differentially expressed in the hybrid at a significant level (P<0.01), we identified 595 up-regulated (22 tags in panicles, 228 in leaves, and 345 in roots) and 25 down-regulated (seven tags in panicles, 15 in leaves, and three in roots) in LYP9. Most of the tag-identified and up-regulated genes were found related to enhancing carbon- and nitrogen-assimilation, including photosynthesis in leaves, nitrogen uptake in roots, and rapid growth in both roots and panicles. Among the down-regulated genes in LYP9, there is an essential enzyme in photorespiration, alanine:glyoxylate aminotransferase 1. Our study adds a new set of data crucial for the understanding of molecular mechanisms of heterosis and gene regulation networks of the cultivated rice.

MeSH Terms
Chromosome Mapping Chromosomes, Plant Expressed Sequence Tags Gene Expression Profiling/methods Gene Expression Regulation, Plant Genome, Plant Hybridization, Genetic Oryza/classification,genetics Species Specificity
Authors & Affiliations
12 authors, click to expand affiliations / ORCID
Bao JingYue
Beijing Genomics Institute, Chinese Academy of Sciences, Beijing 101300, China.
Lee Sanggyu
Chen Chen
Zhang XiuQing
Zhang Yu
Liu SiQi
Clark Terry
Wang Jian
Cao MengLiang
Yang HuanMing
Wang San Ming
Yu Jun
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Article Info
Journal
Plant physiology
Abbr.
Plant Physiol
ISSN
0032-0889
DOI
10.1104/pp.105.060988
Published
2005-07-00
Pages
1216-31
Language
English
Region
United States
NLM ID
0401224
PMCID
PMC1176396
Subset
IM
External Links
PubMed source DOI: 10.1104/pp.105.060988 PMC Full Text View journal details
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