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PMID: 1600938 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

A novel extensin that may organize extracellular matrix biogenesis in Volvox carteri.

The EMBO journal ·Vol. 11 ·No. 6 ·1992-06-00 ·Pages 2055-62

Ertl H, Hallmann A, Wenzl S, Sumper M

Abstract

ISG is a sulphated, extracellular glycoprotein synthesized for only a few minutes in inverting Volvox embryos and inverting sperm cell packets. This control operates at the level of transcription. ISG has been characterized by studies of protein chemistry and electron microscopy. The primary structure of ISG has been derived from genomic DNA and cDNA. ISG is composed of a globular and a rod-shaped domain. The rod-shaped domain represents a member of the extensin family with numerous repeats of Ser-(Hyp)4-6 motifs. A synthetic decapeptide matching the C-terminal sequence is able to disaggregate the organism into individual cells. Immunofluorescence microscopy localizes ISG within the boundary zone of the ECM.

MeSH Terms
Amino Acid Sequence Animals Autoradiography Base Sequence Cloning, Molecular DNA/genetics Eukaryota/genetics,physiology Extracellular Matrix/physiology Glycoproteins/genetics,metabolism,ultrastructure Glycosylation Microscopy, Electron Molecular Sequence Data Oligodeoxyribonucleotides Peptide Fragments/isolation & purification Plant Proteins/metabolism Polymerase Chain Reaction Sulfates/metabolism Sulfur Radioisotopes Transcription, Genetic
Chemicals
Glycoproteins Oligodeoxyribonucleotides Peptide Fragments Plant Proteins Sulfates Sulfur Radioisotopes extensin protein, plant DNA
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Ertl H
Lehrstuhl Biochemie I, Universität Regensburg, FRG.
Hallmann A
Wenzl S
Sumper M
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1992-06-00
Pages
2055-62
Language
English
Region
England
NLM ID
8208664
PMCID
PMC556671
Subset
IM
Databases
GENBANK
X64335, X64555, X64556, X64629, X64700, X65165, X67723, X67724, X67725, X67726
External Links
PubMed source PMC Full Text View journal details
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