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题目:
[Expression of aurora-A kinase in human lung cancer cell lines PG, A549, and NCI-H460].
作者:
Zou(Li-Juan),Li(Guo-Quan),Gong(Lin-Lin),Wang(Yang),Jin(Wei),Zhao(Jin-Yao),Ma(Hai-Ying),Yang(Pei-Man),Shao(Shu-Juan)
状态:
发布时间2005-07-11 , 更新时间 2015-03-13
期刊:
Ai Zheng
摘要:
Recent researches found that Aurora-A overexpresses in various malignancies. This study was to detect the expression of Aurora-A in lung cancer cell lines PG (highly-metastatic giant cell lung cancer), A549 (lung adenocarcinoma), and NCI-H460 (large cell lung cancer) and explore its correlation to DNA content, provide a theoretical basis for screening tumor marker and molecular therapeutic target of lung cancer.,mRNA and protein levels of Aurora-A in PG, A549, and NCI-H460 cells were detected by reverse transcription-polymerase chain reaction(RT-PCR) and Western blot. Flow cytometry was used to analyze DNA contents in cell cycles of PG, A549, and NCI-H460 cells.,mRNA level of Aurora-A was 1.14 in PG cells, 1.16 in A549 cells, and 0.84 in NCI-H460 cells, respectively; protein level of Aurora-A was 8.96 in PG cells, 21.13 in A549 cells, and 6.43 in NCI-H460 cells, respectively. The proportion of cells with tetraploid DNA was 19.88% in PG cells, 14.97% in A549 cells, and 10.6% in NCI-H460 cells, respectively (P<0.01); the proportion of cells with polyploid DNA was 2.66% in PG cells, 3.59% in A549 cells, and 2.30% in NCI-H460 cells, respectively.,Aurora-A is overexpressed in the 3 lung cancer cell lines, but the mRNA levels are different.
语言:
chi
DOI:

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