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PMID: 15996942 Published · ppublish English Letter Research Support, Non-U.S. Gov't

An enzyme-linked immunosorbent assay to screen for inhibitors of the oncogenic anaplastic lymphoma kinase.

Haematologica ·Vol. 90 ·No. 7 ·2005-07-00 ·Pages 988-90

Gunby RH, Tartari CJ, Porchia F, Donella-Deana A, Scapozza L, Gambacorti-Passerini C

Abstract

The discovery of novel anti-cancer drugs targeting anaplastic lymphoma kinase (ALK), an oncogenic tyrosine kinase, raises the need for in vitro assays suitable for screening compounds for ALK inhibition. To this aim we have developed and optimized an ALK-specific enzyme-linked immunosorbent assay that employs a novel ALK peptide substrate and purified ALK kinase domain.

MeSH Terms

Amino Acid Sequence Anaplastic Lymphoma Kinase Animals Dose-Response Relationship, Drug Drug Screening Assays, Antitumor/methods Enzyme Inhibitors/pharmacology Enzyme-Linked Immunosorbent Assay/methods Humans Mice Molecular Sequence Data Peptides/chemistry Phosphorylation Protein Structure, Tertiary Protein-Tyrosine Kinases/antagonists & inhibitors Receptor Protein-Tyrosine Kinases Staurosporine/pharmacology

Chemicals

Enzyme Inhibitors Peptides ALK protein, human Alk protein, mouse Anaplastic Lymphoma Kinase Protein-Tyrosine Kinases Receptor Protein-Tyrosine Kinases Staurosporine

Authors & Affiliations

6 authors, click to expand affiliations / ORCID

Gunby Rosalind Helen

Tartari Carmen Julia

Porchia Francesca

Donella-Deana Arianna

Scapozza Leonardo

Gambacorti-Passerini Carlo

Article Info

Journal

Haematologica

Abbr.

Haematologica

ISSN

1592-8721

Published

2005-07-00

Pages

988-90

Language

English

Region

Italy

NLM ID

0417435

Subset

External Links

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An enzyme-linked immunosorbent assay to screen for inhibitors of the oncogenic anaplastic lymphoma kinase.

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