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PMID: 1599405 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

A biochemical analysis of human periodontal tissue proteoglycans.

The Biochemical journal ·Vol. 284 ( Pt 1) ·1992-05-15 ·Pages 267-74

Larjava H, Häkkinen L, Rahemtulla F

Abstract

Proteoglycans synthesized by periodontal (gingival, periodontal ligament, dental follicle) fibroblasts were analysed by SDS/polyacrylamide and agarose gel electrophoresis after being labelled with radioactive sulphate. Medium, cell membrane and extracellular matrix fractions were analysed separately. Samples were treated with chondroitinase AC, chondroitinase ABC, heparitinase or a combination of chondroitinase ABC and heparitinase before electrophoretic separation of proteoglycans. Antibodies to versican and decorin were used to identify these molecules by Western immunoblots. For steady-state metabolic radiolabelling of fibroblasts, medium and cell membrane fractions contained about equal proportions of radiolabelled proteoglycans (about 43%), whereas less radioactivity (about 14%) was found in proteoglycans of the matrix fraction. Periodontal fibroblasts produce six major proteoglycans: versican, a high-molecular-mass chondroitin sulphate proteoglycan (CSPG); decorin, a dermatan sulphate proteoglycan (DSPG); a membrane-associated heparan sulphate proteoglycan (HSPG); two medium- or matrix-associated HSPGs; and a 91 kDa membrane-associated CSPG. Variation in decorin molecular size was observed in mass cultures of fibroblasts. Similar polydispersity in molecular size of decorin was seen in several clones established from one mass culture.

MeSH Terms
Cell Fractionation Cell Membrane/chemistry Chondroitin Sulfate Proteoglycans/analysis Decorin Electrophoresis, Agar Gel Electrophoresis, Polyacrylamide Gel Extracellular Matrix/chemistry Extracellular Matrix Proteins Fibroblasts/chemistry Heparan Sulfate Proteoglycans Heparitin Sulfate/analysis Humans Lectins, C-Type Periodontium/chemistry,cytology Proteoglycans/analysis Sodium Dodecyl Sulfate Versicans
Chemicals
Chondroitin Sulfate Proteoglycans DCN protein, human Decorin Extracellular Matrix Proteins Heparan Sulfate Proteoglycans Lectins, C-Type Proteoglycans VCAN protein, human Versicans Sodium Dodecyl Sulfate Heparitin Sulfate
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Larjava H
Department of Periodontics, University of Alabama School of Dentistry, Birmingham 35294.
Häkkinen L
Rahemtulla F
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39 references, click to expand
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Article Info
Journal
The Biochemical journal
Abbr.
Biochem J
ISSN
0264-6021
DOI
10.1042/bj2840267
Published
1992-05-15
Pages
267-74
Language
English
Region
England
NLM ID
2984726R
PMCID
PMC1132726
Subset
IM
Grants
NIDCR NIH HHS · 1RO1 DE08466 · United States
NCRR NIH HHS · 2 S07 RR 05300 · United States
NIDCR NIH HHS · R03 DE09134-01 · United States
External Links
PubMed source DOI: 10.1042/bj2840267 PMC Full Text View journal details
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