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PMID: 15968080 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

The Helicobacter pylori CrdRS two-component regulation system (HP1364/HP1365) is required for copper-mediated induction of the copper resistance determinant CrdA.

Journal of bacteriology ·Vol. 187 ·No. 13 ·2005-07-00 ·Pages 4683-8

Waidner B, Melchers K, Stähler FN, Kist M, Bereswill S

Abstract

Here we describe that the Helicobacter pylori sensor kinase produced by HP1364 and the response regulator produced by HP1365 and designated CrdS and CrdR, respectively, are both required for transcriptional induction of the H. pylori copper resistance determinant CrdA by copper ions. CrdRS-deficient mutants lacked copper induction of crdA expression and were copper sensitive. A direct role of CrdR in transcriptional regulation of crdA was confirmed by in vitro binding of CrdR to the crdA upstream region. A 21-nucleotide sequence located near the crdA promoter was shown to be required for CrdR binding.

MeSH Terms
Bacterial Proteins/genetics Base Sequence Binding Sites/genetics Copper/pharmacology Drug Resistance, Bacterial/genetics Genes, Bacterial/genetics Helicobacter pylori/drug effects,genetics Molecular Sequence Data Promoter Regions, Genetic Protein Kinases/genetics,metabolism Trans-Activators/genetics Transcription, Genetic
Chemicals
Bacterial Proteins Trans-Activators osmolarity response regulator proteins Copper Protein Kinases
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Waidner Barbara
Department of Medical Microbiology and Hygiene, Institute of Medical Microbiology and Hygiene, University Hospital Freiburg, Hermann-Herder-Strasse 11, D-79104 Freiburg, Germany. Barbara.Waidner@uniklinik-freiburg.de
Melchers Klaus
Stähler Frank Nils
Kist Manfred
Bereswill Stefan
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/JB.187.13.4683-4688.2005
Email
barbara.waidner@uniklinik-freiburg.de
Published
2005-07-00
Pages
4683-8
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC1151771
Subset
IM
External Links
PubMed source DOI: 10.1128/JB.187.13.4683-4688.2005 PMC Full Text View journal details
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