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PMID: 15958743 Published · ppublish English Journal Article Research Support, N.I.H., Extramural Research Support, U.S. Gov't, P.H.S.

R-type calcium channels contribute to afterdepolarization and bursting in hippocampal CA1 pyramidal neurons.

The Journal of neuroscience : the official journal of the Society for Neuroscience ·Vol. 25 ·No. 24 ·2005-06-15 ·Pages 5763-73

Metz AE, Jarsky T, Martina M, Spruston N

Abstract

Action potentials in pyramidal neurons are typically followed by an afterdepolarization (ADP), which in many cells contributes to intrinsic burst firing. Despite the ubiquity of this common excitable property, the responsible ion channels have not been identified. Using current-clamp recordings in hippocampal slices, we find that the ADP in CA1 pyramidal neurons is mediated by an Ni2+-sensitive calcium tail current. Voltage-clamp experiments indicate that the Ni2+-sensitive current has a pharmacological and biophysical profile consistent with R-type calcium channels. These channels are available at the resting potential, are activated by the action potential, and remain open long enough to drive the ADP. Because the ADP correlates directly with burst firing in CA1 neurons, R-type calcium channels are crucial to this important cellular behavior, which is known to encode hippocampal place fields and enhance synaptic plasticity.

MeSH Terms
Action Potentials/physiology Adenosine Diphosphate/physiology Animals Calcium Channels, R-Type/physiology Hippocampus/physiology In Vitro Techniques Male Membrane Potentials Neuronal Plasticity/physiology Patch-Clamp Techniques Pyramidal Cells/physiology Rats Rats, Wistar Synapses/physiology
Chemicals
Calcium Channels, R-Type Adenosine Diphosphate
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Metz Alexia E
Northwestern University Institute for Neuroscience, Evanston, Illinois 60208, USA.
Jarsky Tim
Martina Marco
Spruston Nelson
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Article Info
Journal
The Journal of neuroscience : the official journal of the Society for Neuroscience
Abbr.
J Neurosci
ISSN
1529-2401
DOI
10.1523/JNEUROSCI.0624-05.2005
Published
2005-06-15
Pages
5763-73
Language
English
Region
United States
NLM ID
8102140
PMCID
PMC6724888
Subset
IM
Grants
NINDS NIH HHS · NS-044688 · United States
NINDS NIH HHS · NS-045437 · United States
NINDS NIH HHS · R01 NS-35180 · United States
External Links
PubMed source DOI: 10.1523/JNEUROSCI.0624-05.2005 PMC Full Text View journal details
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