Home LiteratureArticle Details
PMID: 15951377 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S.

Effect of salicylate on the elasticity, bending stiffness, and strength of SOPC membranes.

Biophysical journal ·Vol. 89 ·No. 3 ·2005-09-00 ·Pages 1789-801

Zhou Y, Raphael RM

Abstract

Salicylate is a small amphiphilic molecule which has diverse effects on membranes and membrane-mediated processes. We have utilized micropipette aspiration of giant unilamellar vesicles to determine salicylate's effects on lecithin membrane elasticity, bending rigidity, and strength. Salicylate effectively reduces the apparent area compressibility modulus and bending modulus of membranes in a dose-dependent manner at concentrations above 1 mM, but does not greatly alter the actual elastic compressibility modulus at the maximal tested concentration of 10 mM. The effect of salicylate on membrane strength was investigated using dynamic tension spectroscopy, which revealed that salicylate increases the frequency of spontaneous defect formation and lowers the energy barrier for unstable hole formation. The mechanical and dynamic tension experiments are consistent and support a picture in which salicylate disrupts membrane stability by decreasing membrane stiffness and membrane thickness. The tension-dependent partitioning of salicylate was utilized to calculate the molecular volume of salicylate in the membrane. The free energy of transfer for salicylate insertion into the membrane and the corresponding partition coefficient were also estimated, and indicated favorable salicylate-membrane interactions. The mechanical changes induced by salicylate may affect several biological processes, especially those associated with membrane curvature and permeability.

MeSH Terms
Animals Anti-Inflammatory Agents, Non-Steroidal/adverse effects,pharmacology Biophysics/methods Cattle Cell Membrane/drug effects,metabolism Dose-Response Relationship, Drug Elasticity Electrochemistry Inflammation Kinetics Lipid Bilayers/chemistry,metabolism Lipids/chemistry Liposomes/metabolism Membranes/metabolism Models, Chemical Models, Statistical Phosphatidylcholines/chemistry Salicylates/chemistry Salicylic Acid/adverse effects,chemistry Serum Albumin/chemistry Spectrophotometry Thermodynamics Time Factors
Chemicals
Anti-Inflammatory Agents, Non-Steroidal Lipid Bilayers Lipids Liposomes Phosphatidylcholines Salicylates Serum Albumin 1-stearoyl-2-oleoyl-sn-glycero-3-phosphocholine Salicylic Acid
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Zhou Yong
Department of Biochemistry and Cell Biology, Rice University, Houston, Texas, USA.
Raphael Robert M
References (54)
54 references, click to expand
  1. Efficacy of antifreeze protein types in protecting liposome membrane integrity depends on phospholipid class.
    Biochim Biophys Acta. 2001 Nov 15;1524(1):11-6 PMID: 11078953
  2. Salicylate ototoxicity; a clinical and experimental study.
    Arch Otolaryngol. 1965 Nov;82(5):483-93 PMID: 4954319
  3. Pinning of fluid membranes by periodic harmonic potentials.
    Phys Rev E Stat Nonlin Soft Matter Phys. 2004 Jan;69(1 Pt 1):011101 PMID: 14995598
  4. Elastic area compressibility modulus of red cell membrane.
    Biophys J. 1976 Jun;16(6):585-95 PMID: 1276386
  5. Water permeability and mechanical strength of polyunsaturated lipid bilayers.
    Biophys J. 2000 Jul;79(1):321-7 PMID: 10866958
  6. Evidence for membrane thinning effect as the mechanism for peptide-induced pore formation.
    Biophys J. 2003 Jun;84(6):3751-8 PMID: 12770881
  7. Luminal surface hydrophobicity of canine gastric mucosa is dependent on a surface mucous gel.
    Gastroenterology. 1990 Feb;98(2):361-70 PMID: 2295391
  8. A membrane bending model of outer hair cell electromotility.
    Biophys J. 2000 Jun;78(6):2844-62 PMID: 10827967
  9. Lipid composition and the lateral pressure profile in bilayers.
    Biophys J. 1999 May;76(5):2625-39 PMID: 10233077
  10. Effect of chain length and unsaturation on elasticity of lipid bilayers.
    Biophys J. 2000 Jul;79(1):328-39 PMID: 10866959
  11. The influence of short-chain alcohols on interfacial tension, mechanical properties, area/molecule, and permeability of fluid lipid bilayers.
    Biophys J. 2004 Aug;87(2):1013-33 PMID: 15298907
  12. Regulation of sodium channel function by bilayer elasticity: the importance of hydrophobic coupling. Effects of Micelle-forming amphiphiles and cholesterol.
    J Gen Physiol. 2004 May;123(5):599-621 PMID: 15111647
  13. Interaction of GM(1) glycolipid in phospholipid monolayers with wheat germ agglutinin: effect of phospholipidic environment and subphase.
    Chem Phys Lipids. 2001 Nov;113(1-2):41-53 PMID: 11687226
  14. Lysolipid exchange with lipid vesicle membranes.
    Ann Biomed Eng. 1995 May-Jun;23(3):287-98 PMID: 7631982
  15. Non-prostaglandin effects of aspirin III and salicylate: inhibition of integrin-dependent human neutrophil aggregation and inflammation in COX 2- and NF kappa B (P105)-knockout mice.
    Adv Exp Med Biol. 2002;507:571-7 PMID: 12664642
  16. Entropy-driven tension and bending elasticity in condensed-fluid membranes.
    Phys Rev Lett. 1990 Apr 23;64(17):2094-2097 PMID: 10041575
  17. Cyclooxygenases 1 and 2.
    Annu Rev Pharmacol Toxicol. 1998;38:97-120 PMID: 9597150
  18. Fractional occurrence of defects in membranes and mechanically driven interleaflet phospholipid transport.
    Phys Rev E Stat Nonlin Soft Matter Phys. 2001 Nov;64(5 Pt 1):051913 PMID: 11735974
  19. Scanning electron microscopy of mucosal biopsies of the human upper gastrointestinal tract.
    Scan Electron Microsc. 1981;4:173-81 PMID: 7347420
  20. Elastic deformation and failure of lipid bilayer membranes containing cholesterol.
    Biophys J. 1990 Oct;58(4):997-1009 PMID: 2249000
  21. Intramembrane electrostatic interactions destabilize lipid vesicles.
    Biophys J. 2002 Oct;83(4):2007-14 PMID: 12324419
  22. The effect of hydrotropic agents on the heat coagulation of bovine serum albumin.
    J Pharm Pharmacol. 1989 May;41(5):298-301 PMID: 2569515
  23. Intracellular anions as the voltage sensor of prestin, the outer hair cell motor protein.
    Science. 2001 Jun 22;292(5525):2340-3 PMID: 11423665
  24. Molecular mechanism of Peptide-induced pores in membranes.
    Phys Rev Lett. 2004 May 14;92(19):198304 PMID: 15169456
  25. Osmotic properties of large unilamellar vesicles prepared by extrusion.
    Biophys J. 1993 Feb;64(2):443-53 PMID: 8457670
  26. Nutrient uptake by protocells: a liposome model system.
    Orig Life Evol Biosph. 2001 Feb-Apr;31(1-2):147-55 PMID: 11296517
  27. Effects of salicylate and lanthanides on outer hair cell motility and associated gating charge.
    J Neurosci. 1996 Aug 15;16(16):4881-9 PMID: 8756420
  28. The effects of gramicidin on electroporation of lipid bilayers.
    Biophys J. 1999 Jun;76(6):3150-7 PMID: 10354439
  29. Thermal fluctuations of large cylindrical phospholipid vesicles.
    Biophys J. 1984 May;45(5):891-9 PMID: 6733240
  30. Aspirin can inhibit gastric mucosal cyclo-oxygenase without causing lesions in rat.
    Gastroenterology. 1983 Apr;84(4):756-61 PMID: 6402412
  31. Thermoelasticity of large lecithin bilayer vesicles.
    Biophys J. 1981 Sep;35(3):637-52 PMID: 7272454
  32. The hydrophobic barrier properties of gastrointestinal mucus.
    Annu Rev Physiol. 1995;57:565-83 PMID: 7778878
  33. Non-steroidal anti-inflammatory drugs (NSAIDs) associate with zwitterionic phospholipids: insight into the mechanism and reversal of NSAID-induced gastrointestinal injury.
    Nat Med. 1995 Feb;1(2):154-8 PMID: 7585013
  34. Aspirin intoxication in a child associated with myocardial necrosis: is this a drug-related lesion?
    Pediatr Dev Pathol. 2003 Jul-Aug;6(4):342-7 PMID: 14692648
  35. Where is the evidence that cyclooxygenase inhibition is the primary cause of nonsteroidal anti-inflammatory drug (NSAID)-induced gastrointestinal injury? Topical injury revisited.
    Biochem Pharmacol. 2001 Mar 15;61(6):631-7 PMID: 11266647
  36. Accelerated interleaflet transport of phosphatidylcholine molecules in membranes under deformation.
    Biophys J. 1996 Sep;71(3):1374-88 PMID: 8874013
  37. Dynamic tension spectroscopy and strength of biomembranes.
    Biophys J. 2003 Oct;85(4):2342-50 PMID: 14507698
  38. Material property characteristics for lipid bilayers containing lysolipid.
    Biophys J. 1998 Jul;75(1):321-30 PMID: 9649389
  39. Nonsteroidal anti-inflammatory drugs and gastroenteropathy: the second hundred years.
    Gastroenterology. 1997 Mar;112(3):1000-16 PMID: 9041264
  40. Thermodynamics of solutions IV: Solvation of ketoprofen in comparison with other NSAIDs.
    J Pharm Sci. 2003 Dec;92(12):2502-11 PMID: 14603496
  41. Interaction of indomethacin and naproxen with gastric surface-active phospholipids: a possible mechanism for the gastric toxicity of nonsteroidal anti-inflammatory drugs (NSAIDs).
    Biochem Pharmacol. 1999 Feb 1;57(3):247-54 PMID: 9890551
  42. Aspirin.
    Sci Am. 1991 Jan;264(1):84-90 PMID: 1899486
  43. Physical principles of membrane organization.
    Q Rev Biophys. 1980 May;13(2):121-200 PMID: 7015403
  44. Excess plasma membrane and effects of ionic amphipaths on mechanics of outer hair cell lateral wall.
    Am J Physiol Cell Physiol. 2002 May;282(5):C1076-86 PMID: 11940523
  45. Inhibition of prostaglandin synthesis as a mechanism of action for aspirin-like drugs.
    Nat New Biol. 1971 Jun 23;231(25):232-5 PMID: 5284360
  46. Does aspirin damage canine gastric mucosa by reducing its surface hydrophobicity?
    Am J Physiol. 1987 Mar;252(3 Pt 1):G421-30 PMID: 3826379
  47. Zwitterionic phospholipids enhance aspirin's therapeutic activity, as demonstrated in rodent model systems.
    J Pharmacol Exp Ther. 1996 Jun;277(3):1221-7 PMID: 8667182
  48. Correlations between changes in indicators of gastric mucosal barrier integrity at time of exposure to "barrier breakers" and extent of hemorrhagic erosions one hour later.
    Dig Dis Sci. 1984 Jan;29(1):6-11 PMID: 6420129
  49. The mechanisms of action of nonsteroidal antiinflammatory drugs.
    Clin Exp Rheumatol. 1989 Sep-Oct;7 Suppl 3:S163-70 PMID: 2557993
  50. Energetics of pore formation induced by membrane active peptides.
    Biochemistry. 2004 Mar 30;43(12):3590-9 PMID: 15035629
  51. Morphological effects of aspirin and prostaglandin on the canine gastric mucosal surface. Analysis with a phospholipid-selective cytochemical stain.
    Gastroenterology. 1990 Mar;98(3):592-606 PMID: 1688815
  52. Pharmacokinetics of drugs in overdose.
    Clin Pharmacokinet. 1992 Aug;23(2):93-105 PMID: 1511533
  53. Ototoxicity of salicylate, nonsteroidal antiinflammatory drugs, and quinine.
    Otolaryngol Clin North Am. 1993 Oct;26(5):791-810 PMID: 8233489
  54. Effects of salicylate on shape, electromotility and membrane characteristics of isolated outer hair cells from guinea pig cochlea.
    Acta Otolaryngol. 1991;111(4):707-18 PMID: 1950533
Article Info
Journal
Biophysical journal
Abbr.
Biophys J
ISSN
0006-3495
DOI
10.1529/biophysj.104.054510
Published
2005-09-00
Epub
2005-00-10
Pages
1789-801
Language
English
Region
United States
NLM ID
0370626
PMCID
PMC1366682
Subset
IM
External Links
PubMed source DOI: 10.1529/biophysj.104.054510 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2