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PMID: 15948257 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Alpha-catenin expression is decreased in patients with gastric carcinoma.

World journal of gastroenterology ·Vol. 11 ·No. 22 ·2005-06-14 ·Pages 3468-72

Zhou YN, Xu CP, Chen Y, Han B, Yang SM, Fang DC

Abstract

To assess the expression of alpha-catenin in gastric carcinoma and to determine the role of alpha-catenin expression in gastric carcinogenesis. alpha-catenin expression was assessed by semi-quantitative reverse transcriptase-polymerase chain reaction and immunohistochemical staining in 49 gastric carcinomas, 26 adjacent non-cancerous mucosae, and gastric biopsy specimens from 11 healthy controls. mRNA levels of alpha-catenin were reduced or absent in 34 of 49 (69%) gastric carcinoma tissues and in 5 of 26 (19%) tumor-free gastric mucosae of carcinoma patients, respectively. Of the carcinoma samples with altered alpha-catenin mRNA levels, alpha-catenin expression was negative in 20 and decreased in 14 cases. Up to 69% of tumors were stained abnormally for alpha-catenin. Of the 34 cases whose mRNA expression of alpha-catenin was reduced, 32 (94%) showed abnormal immunostaining patterns, while only 2 showed a normal alpha-catenin expression. The frequency of reduced expression of alpha-catenin mRNA was 14% in well-differentiated carcinomas, higher than that in poorly differentiated carcinomas (86%). A significant correlation was not shown between alpha-catenin expression and both depth of invasion and lymph node metastasis. Moreover, there was no statistical difference between loss or down-regulation of alpha-catenin mRNA and Helicobacter pylori (H. pylori) infection. Downregulation of alpha-catenin expression is common in gastric carcinoma, and alpha-catenin expression may be used as a differentiation marker. Downregulation of alpha-catenin expression may be an early event in tumorigenesis. Reduced alpha-catenin expression is not correlated with H. pylori infection.

MeSH Terms
Adult Aged Cytoskeletal Proteins/genetics,metabolism Female Gene Expression Regulation, Neoplastic Helicobacter Infections/metabolism,pathology,physiopathology Humans Male Middle Aged Neoplasm Staging Stomach Neoplasms/metabolism,pathology,physiopathology alpha Catenin
Chemicals
CTNNA1 protein, human Cytoskeletal Proteins alpha Catenin
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Zhou Yong-Ning
Department of Gastroenterology, First Hospital, Lanzhou University, Lanzhou 730000, Gansu Province, China. yongningzhou@sina.com.cn
Xu Cai-Pu
Chen Yu
Han Biao
Yang Shi-Ming
Fang Dian-Chun
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Article Info
Journal
World journal of gastroenterology
Abbr.
World J Gastroenterol
ISSN
1007-9327
DOI
10.3748/wjg.v11.i22.3468
Email
yongningzhou@sina.com.cn
Published
2005-06-14
Pages
3468-72
Language
English
Region
United States
NLM ID
100883448
PMCID
PMC4316006
Subset
IM
External Links
PubMed source DOI: 10.3748/wjg.v11.i22.3468 PMC Full Text View journal details
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