| GeneLibs

题目:: Increase of bone marrow-derived secretory lineage epithelial cells during regeneration in the human intestine.
作者:: Matsumoto(Tomoko),Okamoto(Ryuichi),Yajima(Tomoharu),Mori(Takehiko),Okamoto(Shinichiro),Ikeda(Yasuo),Mukai(Makio),Yamazaki(Motomi),Oshima(Shigeru),Tsuchiya(Kiichiro),Nakamura(Tetsuya),Kanai(Takanori),Okano(Hideyuki),Inazawa(Johji),Hibi(Toshifumi),Watanabe(Mamoru)
状态:: 发布时间2005-06-07 , 更新时间 2006-11-15
期刊:: Gastroenterology
摘要:: We have previously reported that bone marrow (BM)-derived cells contribute to the regeneration of the human intestinal epithelium. To analyze further how these cells arise, proliferate, and differentiate as epithelial cells, histologic analysis was conducted using endoscopic specimens.,Thirty biopsy specimens from 14 female, sex-mismatched BM-transplantation recipients were examined. BM-derived cells were identified by fluorescent in situ hybridization (FISH) for the Y chromosome and immunohistochemistry. Multicolor FISH was used to exclude cell fusion. These cells were further analyzed for various differentiation or proliferation markers.,No evidence of cell fusion was detected. BM-derived cells did not distribute within the crypt as stem cells and rarely expressed Musashi-1. However, BM-derived epithelial cells frequently expressed Ki-67, and some of these cells appeared as pairs of adjacent cells. These cells also expressed markers of all 4 lineages of terminally differentiated cells. During regeneration following graft-vs-host disease, the number of BM-derived cells was substantially increased within Ki-67-positive cells. Interestingly, the number of cells expressing markers for secretory lineage cells was significantly increased within BM-derived cells. This change was unique for BM-derived cells, resulting in a significantly increased proportion of BM-derived cells among secretory lineage cells.,BM-derived epithelial cells arise via a mechanism other than cell fusion and rarely give rise to stem cells. However, a small proportion of these cells express proliferation markers, and a majority reside as terminally differentiated cells. During regeneration BM-derived cells increase as secretory lineage cells, thereby contributing to restore epithelial functions.
语言:: eng
DOI:

文献库文献相关信息

DataTable pData

联系方式

微信公众号

文献库 文献相关信息

DataTable pData

联系方式

微信公众号

文献库文献相关信息