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LiteratureArticle Details
PMID: 15933159
Published · ppublish
English
Historical Article
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.
Research Support, U.S. Gov't, P.H.S.
Genomic sequencing of Pleistocene cave bears.
Abstract
Despite the greater information content of genomic DNA, ancient DNA studies have largely been limited to the amplification of mitochondrial sequences. Here we describe metagenomic libraries constructed with unamplified DNA extracted from skeletal remains of two 40,000-year-old extinct cave bears. Analysis of approximately 1 megabase of sequence from each library showed that despite significant microbial contamination, 5.8 and 1.1% of clones contained cave bear inserts, yielding 26,861 base pairs of cave bear genome sequence. Comparison of cave bear and modern bear sequences revealed the evolutionary relationship of these lineages. The metagenomic approach used here establishes the feasibility of ancient DNA genome sequencing programs.
MeSH Terms
Animals
Cloning, Molecular
Computational Biology
DNA/genetics,history
Dogs/genetics
Genome
Genomic Library
History, Ancient
Molecular Sequence Data
Phylogeny
Sequence Alignment
Sequence Analysis, DNA
Ursidae/genetics
Chemicals
DNA
Article Info
Journal
Science (New York, N.Y.)
Abbr.
Science
ISSN
1095-9203
Published
2005-07-22
Epub
2005-00-02
Pages
597-9
Language
English
Region
United States
NLM ID
0404511
Subset
IM
Grants
NHLBI NIH HHS · T32 HL07279 · United States
NHLBI NIH HHS · U1 HL66681B · United States
Databases
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